Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity

doi:10.1007/s00343-018-7089-6

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	Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity
	Sun Yuhao1,2 ; Chen Xiaolin 1; Liu Song 1; Yu Huahua1 ; Li Rongfeng 1; Wang Xueqin 1; Qin Yukun1 ; Li Pengcheng1
	2018-05-01
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
卷号	36 期号:3 页码:882-891
通讯作者	Chen Xiaolin([email protected]) ; Li Pengcheng([email protected])
摘要	Heparin has been used as an anticoagulant drug for many years, but it has significant side effects. In the search far good substitutes, low molecular weight (MW) polysaccharides from Sargassum fusiforme have been examined and confirmed to possess biological activities. Here, S. fusiforme polysaccharides (SFP) were extracted and subjected to a hydrogen peroxide (H2O2) oxidation method for the preparation of low-MW SFP (LSFP). The effects of temperature, pH, and H2O2 concentration on the degradation process were also examined. Several LSFP of 36, 9, 5.7, and 2.7 kDa were obtained under different conditions, and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin (APTT), prothrombin (PT) and thrombin times (TT) significantly, indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic, extrinsic, and common coagulation pathways. As these effects were related to the MW of the polysaccharides in APTT and TT but not in PT, the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined effects. The details of this mechanism require further verification.
关键词	anticoagulant activity Sargassum fusiforme low molecular weight degradation
DOI	10.1007/s00343-018-7089-6
收录类别	SCI
语种	英语
资助项目	Ocean Public Welfare Scientific Research Project, State Oceanic Administration of the People's Republic of China[201505033]###3373; Qingdao People's Livelihood Science and Technology Program[16-6-2-41-nsh]###3374; Ocean Public Welfare Scientific Research Project, State Oceanic Administration of the People's Republic of China[201505033]; Qingdao People's Livelihood Science and Technology Program[16-6-2-41-nsh]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000438791100026
出版者	SCIENCE PRESS
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163508
专题	中国科学院海洋研究所
通讯作者	Chen Xiaolin; Li Pengcheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun Yuhao,Chen Xiaolin,Liu Song,et al. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(3):882-891.
APA	Sun Yuhao.,Chen Xiaolin.,Liu Song.,Yu Huahua.,Li Rongfeng.,...&Li Pengcheng.(2018).Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(3),882-891.
MLA	Sun Yuhao,et al."Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.3(2018):882-891.

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