The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas

doi:10.1007/s10126-018-9806-8

IOCAS-IR

	The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas
	Wei, Lei 1; Xu, Fei 2; Wang, Yuzhi 3; Cai, Zhongqiang 4; Yu, Wenchao 1; He, Cheng 1; Jiang, Qiuyun 1; Xu, Xiqiang 5; Guo, Wen 6; Wang, Xiaotong 1
	2018-08-01
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
卷号	20 期号:4 页码:425-435
摘要	Left-right (L-R) asymmetry is controlled by gene regulation pathways for the L-R axis, and in vertebrates, the gene Pitx2 in TGF-beta signaling pathway plays important roles in the asymmetrical formation of organs. However, less is known about the asymmetries of anatomically identical paired organs, as well as the transcriptional regulation mechanism of the gene Pitx in invertebrates. Here, we report the molecular biological differences between the left and right mantles of an invertebrate, the Pacific oyster Crassostrea gigas, and propose one possible mechanism underlying those differences. RNA sequencing (RNA-seq) analysis indicated that the paired organs showed different gene expression patterns, suggesting possible functional differences in shell formation, pheromone signaling, nerve conduction, the stress response, and other physiological processes. RNA-seq and real-time qPCR analysis indicated high right-side expression of the Pitx homolog (cgPitx) in oyster mantle, supporting a conserved role for Pitx in controlling asymmetry. Methylation-dependent restriction-site associated DNA sequencing (MethylRAD) identified a methylation site in the promoter region of cgPitx and showed significantly different methylation levels between the left and right mantles. This is the first report, to our knowledge, of such a difference in methylation in spiralians, and it was further confirmed in 18 other individuals by using a pyrosequencing assay. The miRNome analysis and the TGF-beta receptor/Smad inhibition experiment further supported that several genes in TGF-beta signaling pathway may be related with the L/R asymmetry of oyster mantles. These results suggested that the molecular differentiation of the oyster's paired left and right mantles is significant, TGF-beta signaling pathway could be involved in establishing or maintaining the asymmetry, and the cgPitx gene as one of genes in this pathway; the different methylation levels in its promoter regions between L/R mantles was the one of possible mechanisms regulating the left-right functional differentiation.
关键词	Pacific oyster Asymmetry of paired organs Mantle The gene Pitx Methylation Promoter region
DOI	10.1007/s10126-018-9806-8
收录类别	SCI
语种	英语
资助项目	Project of Shandong Province Higher Educational Science and Technology Program[J17KA129]###3359; Shandong Provincial Natural Science Foundation, China[ZR2017BC058]###3358; Shandong Provincial Natural Science Foundation, China[ZR2013CM026]###3357; National Natural Science Foundation of China[31302181]###3356; Innovation Plan for Marine/Fishery Science and Technology of Shandong Province, China[2017YY03]###3355; Key R & D Program of Yantai City, China[2017ZH054]###3354; Modern Agricultural Industry Technology System of Shandong Province, China[SDAIT-14-03]###3353; National Natural Science Foundation of China[41776152]###790; National Natural Science Foundation of China[41776152]; Modern Agricultural Industry Technology System of Shandong Province, China[SDAIT-14-03]; Key R & D Program of Yantai City, China[2017ZH054]; Innovation Plan for Marine/Fishery Science and Technology of Shandong Province, China[2017YY03]; National Natural Science Foundation of China[31302181]; Shandong Provincial Natural Science Foundation, China[ZR2013CM026]; Shandong Provincial Natural Science Foundation, China[ZR2017BC058]; Project of Shandong Province Higher Educational Science and Technology Program[J17KA129]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000439341200001
出版者	SPRINGER
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/163484
专题	中国科学院海洋研究所
通讯作者	Wang, Xiaotong
作者单位	1.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Shandong Univ, Sch Life Sci, Jinan 250100, Shandong, Peoples R China 4.Chinese Acad Fishery Sci, Changdao Enhancement & Expt Stn, Changdao 265800, Peoples R China 5.Qingdao OE Biotechnol Co Ltd, Qingdao 266101, Peoples R China 6.Marine Biol Inst Shandong Prov, Qingdao 266104, Peoples R China
推荐引用方式 GB/T 7714	Wei, Lei,Xu, Fei,Wang, Yuzhi,et al. The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas[J]. MARINE BIOTECHNOLOGY,2018,20(4):425-435.
APA	Wei, Lei.,Xu, Fei.,Wang, Yuzhi.,Cai, Zhongqiang.,Yu, Wenchao.,...&Wang, Xiaotong.(2018).The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas.MARINE BIOTECHNOLOGY,20(4),425-435.
MLA	Wei, Lei,et al."The Molecular Differentiation of Anatomically Paired Left and Right Mantles of the Pacific Oyster Crassostrea gigas".MARINE BIOTECHNOLOGY 20.4(2018):425-435.

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