COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)

	COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)
	Lyu, Man1,3 ; Lei, Yanli1,3 ; Li, Tiegang2,3,4
	2018-07-01
发表期刊	JOURNAL OF FORAMINIFERAL RESEARCH
ISSN	0096-1191
卷号	48 期号:3 页码:186-192
通讯作者	Lei, Yanli([email protected]) ; Li, Tiegang()
摘要	We carried out a series of experiments to evaluate the efficiency of preserving DNA from porcelaneous foraminifera (Quinqueloculina spp.) and a second set to assess the effect of Rose Bengal staining on molecular processing. The first experimental setup assessed three methods of DNA preservation (air-drying, freezing with or without seawater, and Guanidine lysis buffer treatment with or without EDTA (Ethylenediaminetetraacetic acid)). Our study produced the following results: 1) there were no significant differences in DNA preservation when samples were air dried across a range of temperatures (20-120 degrees C); samples frozen at -20 degrees C appeared better preserved than at those frozen at -80 degrees C, and freezing without seawater appeared to produce better preservation than with seawater, though differences in freezing treatments were not significant (p>0.05); samples in Guanidine lysis buffer with EDTA and stored at -20 degrees C were well preserved (p<0.05); 2) sometimes, DNA was successfully extracted from samples stained with Rose Bengal. We recommend Guanidine lysis buffer with EDTA, stored at -20 degrees C for up to six weeks, as the best protocol for preservation of DNA from porcelaneous foraminifera.
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11030104]###488; National Science Foundation of China[41476043]###3319; National Science Foundation of China[41230959]###1490; National Program on 'Global Change and Air-Sea Interaction'[GASI-GEOGE-06-02]###3320; Continental Shelf Drilling Program of China[GZH201100202]###144; Paul Bronnimann Foundation###490; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11030104]; National Science Foundation of China[41476043]; National Science Foundation of China[41230959]; National Program on 'Global Change and Air-Sea Interaction'[GASI-GEOGE-06-02]; Continental Shelf Drilling Program of China[GZH201100202]; Paul Bronnimann Foundation
WOS研究方向	Paleontology
WOS类目	Paleontology
WOS记录号	WOS:000439322600002
出版者	ALLEN PRESS INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163450
专题	海洋生物分类与系统演化实验室
通讯作者	Lei, Yanli; Li, Tiegang
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 2.SOA, Inst Oceanog 1, Key Lab Marine Sedimentol & Environm Geol, Qingdao 266061, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao 266061, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Lyu, Man,Lei, Yanli,Li, Tiegang. COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)[J]. JOURNAL OF FORAMINIFERAL RESEARCH,2018,48(3):186-192.
APA	Lyu, Man,Lei, Yanli,&Li, Tiegang.(2018).COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA).JOURNAL OF FORAMINIFERAL RESEARCH,48(3),186-192.
MLA	Lyu, Man,et al."COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)".JOURNAL OF FORAMINIFERAL RESEARCH 48.3(2018):186-192.