A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei

doi:10.1016/j.dci.2019.103491

IOCAS-IR > 实验海洋生物学重点实验室

	A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei
	Yang, Feifei 1; Li, Xuechun1,2,3,4 ; Li, Shihao 1,2,3; Xiang, Jianhai1,2,3 ; Li, Fuhua 1,2,3
	2020
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	102 页码:9
摘要	As the most productive crustacean species in aquaculture, Litopenaeus vannamei is seriously threatened by white spot syndrome virus (WSSV), which has caused huge economic damage in the past decades. Shrimp cuticle proteins are the important components in the frontier target tissues, including cuticle and the chitinous lining of the digestive tract. In present study, a novel cuticle protein gene, named LvCPAP1, was isolated and demonstrated to play an important role in WSSV infection. The deduced amino acid sequence of LvCPAP1 contained a signal peptide and a conserved chitin-binding domain type 2 (ChBD2). Tissue distribution analysis revealed that LvCPAP1 was predominantly expressed in epidermis and stomach. The transcription levels of LvCPAP1 in epidermis and stomach were significantly regulated upon WSSV challenge. DsRNA silencing of LvCPAP1 decreased the in vivo WSSV copy numbers and the death rate of shrimp after WSSV infection, indicating that LvCPAP1 might facilitate WSSV invasion. In addition, the interaction between LvCPAP1 and the major envelop protein VP24 of WSSV was revealed by yeast two-hybrid system and further confirmed by dot blot and pull-down assays. The present study implied that cuticle protein LvCPAP1 might favor the entry process of WSSV, which provided new clues for understanding the role of cuticle proteins during virus infection.
关键词	Litopenaeus vannamei WSSV Cuticle protein Chitin binding domain RNAi Interaction
DOI	10.1016/j.dci.2019.103491
收录类别	SCI
语种	英语
资助项目	Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017N004]###3144; China Postdoctoral Science Foundation[2017M612361]###1409; China Agriculture Research System-48[CARS-48]###1408; China Agriculture Research System-48[CARS-48]; China Postdoctoral Science Foundation[2017M612361]; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017N004]
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000491213900020
出版者	ELSEVIER SCI LTD
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/163435
专题	实验海洋生物学重点实验室中国科学院海洋大科学研究中心
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yang, Feifei,Li, Xuechun,Li, Shihao,et al. A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,102:9.
APA	Yang, Feifei,Li, Xuechun,Li, Shihao,Xiang, Jianhai,&Li, Fuhua.(2020).A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,102,9.
MLA	Yang, Feifei,et al."A novel cuticle protein involved in WSSV infection to the Pacific white shrimp Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 102(2020):9.

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A novel cuticle prot（1914KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览