IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)

doi:10.1016/j.fsi.2019.07.074

IOCAS-IR > 实验海洋生物学重点实验室

	IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)
	Huang, Baoyu1,3,4,5 ; Tang, Xueying 1,3,4,5; Zhang, Linlin 1,3,5; Li, Li 1,2,4,5; Wang, Wei 1,2,4,5; Liu, Mingkun 1,4,5; Zhang, Guofan 1,3,4,5
	2019-10-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	93 页码:551-558
通讯作者	Li, Li([email protected]) ; Zhang, Guofan([email protected])
摘要	IxB-related kinase epsilon (IKK epsilon) plays a crucial role in the activation of nuclear factor kappa B (NF-kappa B) by phosphorylating inhibitor of NF-kappa B (I kappa B) and in the regulation of interferon (IFN) gene expression by phosphorylating IFN regulatory factors (IRFs). In this study, we cloned an IKK epsilon homologue cDNA (designated as CgIKK epsilon-like) from the Pacific oyster, Crassostrea gigas. The full 2896-bp cDNA sequence comprised a 2163-bp open reading frame (ORF) encoding 720 amino acids. CgIKK epsilon-like is ubiquitously expressed, and its mRNA levels in hemocytes after poly I:C, V. alginolyticus, or OsHV-1 mu Var challenge were analyzed by real-time PCR. Compared to that in the control, CgIKK epsilon-like mRNA expression levels were significantly increased at 3 h and peaked at 6 h after OsHV-1 mu Var challenge; no obvious changes were observed in expression levels until 24 h after either V. alginolyticus or poly I:C challenge, reaching a maximum at 24 h (p < 0.01) and then rapidly decreasing. CgIKK epsilon-like transfection into human cell lines induced NF-kappa B and ISRE activation, while transfection with CgIKK epsilon-like deletion mutants abolished NF-kappa B and ISRE reporter gene activation. Additionally, CgIKK epsilon-like could interact with CgTBK1 and could form homodimers strongly, which may be critical for the immune signaling transduction. Last but not least, we found that CgIKK epsilon-like may increase CgI kappa Bs phosphorylation and could interact with CgIRF8. Together, these results suggest that CgIKK epsilon-like could respond to pathogenic infection, participate in the immune signal transduction and activate NF-kappa B and ISRE reporter genes. Thus, CgIKK epsilon-like could play an important role in the oyster immune system.
关键词	Oyster Innate immunity IKKe TBK1 NF-kappa B IRF
DOI	10.1016/j.fsi.2019.07.074
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31802328]###2894; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science[MS2018NO02]###3239; earmarked fund for Modern Agro-industry Technology Research System[CARS-49]###157; National Natural Science Foundation of China[31802328]; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science[MS2018NO02]; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000488998300061
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163251
专题	实验海洋生物学重点实验室
通讯作者	Li, Li; Zhang, Guofan
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Huang, Baoyu,Tang, Xueying,Zhang, Linlin,et al. IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:551-558.
APA	Huang, Baoyu.,Tang, Xueying.,Zhang, Linlin.,Li, Li.,Wang, Wei.,...&Zhang, Guofan.(2019).IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas).FISH & SHELLFISH IMMUNOLOGY,93,551-558.
MLA	Huang, Baoyu,et al."IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)".FISH & SHELLFISH IMMUNOLOGY 93(2019):551-558.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Huang-2019-Ikk-like-（2123KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览
IKK epsilon-like pla（2123KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览