Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum | |
Wu, Songcui1,2,3 ; Gu, Wenhui1,2,3; Huang, Aiyou1,2,3; Li, Yuanxiang1,2,3; Kumar, Manoj4; Lim, Phaik Eem5; Huan, Li1,2,3; Gao, Shan1,2,3; Wang, Guangce1,2,3
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| 2019-09-23 | |
| 发表期刊 | MICROBIAL CELL FACTORIES
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| 卷号 | 18期号:1页码:16 |
| 通讯作者 | Wang, Guangce([email protected]) |
| 摘要 | Background Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutum, along with an enhancement of the oxidative pentose phosphate pathway (OPPP) activity. The purpose of this work directed toward the verification of the critical role of glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme in the OPPP, in lipid accumulation in P. tricornutum and its simultaneous rapid growth rate under high-CO2 (0.15%) cultivation. Results In this study, G6PDH was identified as a target for algal strain improvement, wherein G6PDH gene was successfully overexpressed and antisense knockdown in P. tricornutum, and systematic comparisons of the photosynthesis performance, algal growth, lipid content, fatty acid profiles, NADPH production, G6PDH activity and transcriptional abundance were performed. The results showed that, due to the enhanced G6PDH activity, transcriptional abundance and NAPDH production, overexpression of G6PDH accompanied by high-CO2 cultivation resulted in a much higher of both lipid content and growth in P. tricornutum, while knockdown of G6PDH greatly decreased algal growth as well as lipid accumulation. In addition, the total proportions of saturated and unsaturated fatty acid, especially the polyunsaturated fatty acid eicosapentaenoic acid (EPA; C20:5, n-3), were highly increased in high-CO2 cultivated G6PDH overexpressed strains. Conclusions The successful of overexpression and antisense knockdown of G6PDH well demonstrated the positive influence of G6PDH on algal growth and lipid accumulation in P. tricornutum. The improvement of algal growth, lipid content as well as polyunsaturated fatty acids in high-CO2 cultivated G6PDH overexpressed P. tricornutum suggested this G6PDH overexpression-high CO2 cultivation pattern provides an efficient and economical route for algal strain improvement to develop algal-based biodiesel production. |
| 关键词 | Glucose-6-phosphate dehydrogenase Overexpression Antisense knockdown CO2 Phaeodactylum tricornutum Lipid accumulation Algal growth rate |
| DOI | 10.1186/s12934-019-1214-x |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[41806171]###2949; National Natural Science Foundation of China[41876158]###2948; National Key R&D Program of China[2018YFD0901501-1]###2950; Haikou Marine Economic Demonstration City Industrial Chain Collaborative Innovation Project[HHCL201811]###3190; Tianjin Marine Economic Demonstration[BHSF2017-21]###2952; National Natural Science Foundation of China[41806171]; National Natural Science Foundation of China[41876158]; National Key R&D Program of China[2018YFD0901501-1]; Haikou Marine Economic Demonstration City Industrial Chain Collaborative Innovation Project[HHCL201811]; Tianjin Marine Economic Demonstration[BHSF2017-21] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000487442400002 |
| 出版者 | BMC |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/162957 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 4.UTS, Fac Sci, Climate Change Cluster, Sydney, NSW, Australia 5.Univ Malaya, IOES, Kuala Lumpur 50603, Malaysia |
| 第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Wu, Songcui,Gu, Wenhui,Huang, Aiyou,et al. Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum[J]. MICROBIAL CELL FACTORIES,2019,18(1):16. |
| APA | Wu, Songcui.,Gu, Wenhui.,Huang, Aiyou.,Li, Yuanxiang.,Kumar, Manoj.,...&Wang, Guangce.(2019).Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum.MICROBIAL CELL FACTORIES,18(1),16. |
| MLA | Wu, Songcui,et al."Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum".MICROBIAL CELL FACTORIES 18.1(2019):16. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Wu-2019-Elevated-co-（2841KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
| Elevated CO2 improve（2841KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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