| Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi | |
| Zhao, Jin1,2; Wei, Jianteng3; Liu, Ming1; Xiao, Lin1,2; Wu, Ning1; Liu, Ge1,2; Huang, Haijuan4; Zhang, Yuyan4; Zheng, Lanhong5,6; Lin, Xiukun1,6; Lin, XK | |
| 2013-08-01 | |
| 发表期刊 | BIOCHIMIE
 |
| ISSN | 0300-9084 |
| 卷号 | 95期号:8页码:1611-1619 |
| 文章类型 | Article |
| 摘要 | Previous study in our laboratory confirmed that a novel polypeptide, CS5931 derived from Ciona savignyi possesses potent antitumor activity. In the present study, the full length cDNA of CS5931 precursor, termed Cs-pgrn-1 was cloned. The complete cDNA sequence of this gene consists of 685 bp containing an open reading frame (ORF) of 522 bp (173 amino acid residues). In silico analysis revealed that the polypeptide consists of two identical domains, similar with granulin (GRN) found in other species, and each of the domain encodes a polypeptide identical with CS5931. Phylogenetic analysis confirmed that CS5931 shares high homology with Ciona intestinalis GRN and is conserved during evolution. The polypeptide also shows high similarity with human GRN A, B, and C. Prediction of 3D protein structure revealed the 3D structure of CS5931 is very similar with human GRN A. The CS5931 was expressed using a prokaryotic expression system and the purified polypeptide inhibited the growth of several tumor cell lines in vitro via apoptotic pathway. Our study revealed that CS5931 has the potential to be developed as a novel antitumor agent. (C) 2013 Elsevier Masson SAS. All rights reserved.; Previous study in our laboratory confirmed that a novel polypeptide, CS5931 derived from Ciona savignyi possesses potent antitumor activity. In the present study, the full length cDNA of CS5931 precursor, termed Cs-pgrn-1 was cloned. The complete cDNA sequence of this gene consists of 685 bp containing an open reading frame (ORF) of 522 bp (173 amino acid residues). In silico analysis revealed that the polypeptide consists of two identical domains, similar with granulin (GRN) found in other species, and each of the domain encodes a polypeptide identical with CS5931. Phylogenetic analysis confirmed that CS5931 shares high homology with Ciona intestinalis GRN and is conserved during evolution. The polypeptide also shows high similarity with human GRN A, B, and C. Prediction of 3D protein structure revealed the 3D structure of CS5931 is very similar with human GRN A. The CS5931 was expressed using a prokaryotic expression system and the purified polypeptide inhibited the growth of several tumor cell lines in vitro via apoptotic pathway. Our study revealed that CS5931 has the potential to be developed as a novel antitumor agent. (C) 2013 Elsevier Masson SAS. All rights reserved. |
| 关键词 | Ciona Savignyi Granulin Antitumor Polypeptide Apoptosis |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1016/j.biochi.2013.05.001 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000321801500012 |
| WOS关键词 | EPITHELIN/GRANULIN PRECURSOR EXPRESSION ; EPITHELIAL-CELL GROWTH ; FISH CYPRINUS-CARPIO ; BREAST-CANCER CELLS ; 2 BETA-HAIRPINS ; GRANULIN/EPITHELIN PRECURSOR ; HEPATOCELLULAR-CARCINOMA ; CARASSIUS-AURATUS ; EMERGING FAMILY ; FACTOR PCDGF |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16294 |
| 专题 | 海洋地质与环境重点实验室 |
| 通讯作者 | Lin, XK |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 4.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China 5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 6.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Jin,Wei, Jianteng,Liu, Ming,et al. Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi[J]. BIOCHIMIE,2013,95(8):1611-1619. |
| APA | Zhao, Jin.,Wei, Jianteng.,Liu, Ming.,Xiao, Lin.,Wu, Ning.,...&Lin, XK.(2013).Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi.BIOCHIMIE,95(8),1611-1619. |
| MLA | Zhao, Jin,et al."Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi".BIOCHIMIE 95.8(2013):1611-1619. |
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| Cloning, characteriz（2060KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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