Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity

doi:10.1016/j.cbpb.2019.110310

IOCAS-IR > 实验海洋生物学重点实验室

	Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity
	Tan, Xungang1,2 ; Xu, Peng1,2,3 ; Zhang, Yuqing 1,2,3; Zhang, Pei-Jun 1,2
	2019-10-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	236 页码:9
通讯作者	Tan, Xungang([email protected])
摘要	Myogenic regulatory factor 4 (MRF4) is a basic helix-loop-helix (bHLH) transcription factor that plays crucial roles in myoblast differentiation and maturation. Here, we report the isolation of the olive flounder (Paralichthys olivaceus) mrf4 gene and the spatiotemporal analysis of its expression patterns. Sequence analysis indicated that flounder mrf4 shared a similar structure with other vertebrate MRF4, including the conserved bHLH domain. Flounder mrf4 contains 3 exons and 2 introns. Sequence alignment and phylogenetic analysis showed that it was highly homologous with Salmo salar, Danio rerio, Takifugu rubripes, and Tetraodon nigroviridis mrf4. Flounder mrf4 was first expressed in the medial region of somites that give rise to slow muscles, and later spread to the lateral region of somites that give rise to fast muscles. Mrf4 transcript levels decreased significantly in mature somites in the trunk region, and expression could only be detected in the caudal somites, consistent with the timing of somite maturation. Transient expression analysis showed that the 506 bp flounder mrf4 promoter was sufficient to direct muscle-specific GFP expression in zebrafish embryos.
关键词	Olive flounder Myogenesis Somites mrf4 promoter Zebrafish
DOI	10.1016/j.cbpb.2019.110310
收录类别	SCI
语种	英语
资助项目	National Sciences of Foundation of China[31672636]###3051; National Sciences of Foundation of China[31128017]###3052; National Sciences of Foundation of China[31672636]; National Sciences of Foundation of China[31128017]
WOS研究方向	Biochemistry & Molecular Biology ; Zoology
WOS类目	Biochemistry & Molecular Biology ; Zoology
WOS记录号	WOS:000484655000003
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162703
专题	实验海洋生物学重点实验室
通讯作者	Tan, Xungang
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Tan, Xungang,Xu, Peng,Zhang, Yuqing,et al. Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2019,236:9.
APA	Tan, Xungang,Xu, Peng,Zhang, Yuqing,&Zhang, Pei-Jun.(2019).Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,236,9.
MLA	Tan, Xungang,et al."Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 236(2019):9.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Tan-2019-Olive-floun（3179KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览
Olive flounder (Para（3179KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览