Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins

doi:10.1007/s10811-019-1735-z

IOCAS-IR > 实验海洋生物学重点实验室

	Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins
	Zhao, Ping 1,2,3,4; Niu, Jianfeng 1,2,4; Huan, Li1,2,4 ; Gu, Wenhui 1,2,4; Wu, Mingjiang 5; Wang, Guangce 1,2,4
	2019-08-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	31 期号:4 页码:2497-2505
通讯作者	Wang, Guangce([email protected])
摘要	Pyropia haitanensis is one of the most important and commercial species of edible seaweed in China. However, at its late growth stages it is unsuitable for food processing and is usually treated as waste. The present study investigated the full use of aging Py. haitanensis and attempted to improve its economic value through the purification of R-phycoerythrin combining the extraction of agar from the dry residue after phycobiliprotein extraction. The recovery of R-phycoerythrin from crude extract was 12.12% with a purification ratio of > 4. The agar yield was 5.81% with a gel strength of 796.00 +/- 28.10 g cm(-2), a gelling temperature of 38.10 +/- 0.22 degrees C, a melting temperature of 89.50 +/- 0.55 degrees C, 3,6-anhydrogalactose content of 34% +/- 3.16%, and sulfate content of 3.01% +/- 0.24%. There were no significant differences between these parameters and those from the direct extraction of Py. haitanensis. The agar extracted exhibited some physicochemical properties that have industrial applications. It could also be used as a material for DNA electrophoresis and a bacterial culture medium. This new method of comprehensive extraction reduced use of water and alkali, and waste liquid output, which were 36.52 L, 0.40 L, and 39.15 L, respectively, less than that from direct Py. haitanensis extraction. Total nitrogen, suspended solids, phosphorus, ammonia-nitrogen, chemical oxygen demand, and biochemical oxygen demand of the extract after 5 days were 38.37 mg, 4.72 x 10(3) mg, 62.82 mg, 18.32 mg, 8.46 x 10(3) mg, and 2.21 x 10(3) mg, respectively, less than that directly from Py. haitanensis.
关键词	Pyropia haitanensis Expanded bed adsorption R-phycoerythrin Agar
DOI	10.1007/s10811-019-1735-z
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2016YFC1400606]###3007; Natural Science Foundation of China[31470430]###3008; Natural Science Foundation of China[41876197]###3009; National Key Research and Development Program of China[2016YFC1400606]; Natural Science Foundation of China[31470430]; Natural Science Foundation of China[41876197]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000479057200030
出版者	SPRINGER
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162364
专题	实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 5.Wenzhou Univ, Coll Life & Environm Sci, Wenzhou, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhao, Ping,Niu, Jianfeng,Huan, Li,et al. Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2497-2505.
APA	Zhao, Ping,Niu, Jianfeng,Huan, Li,Gu, Wenhui,Wu, Mingjiang,&Wang, Guangce.(2019).Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins.JOURNAL OF APPLIED PHYCOLOGY,31(4),2497-2505.
MLA	Zhao, Ping,et al."Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2497-2505.

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