A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts

doi:10.1039/c9mo00025a

IOCAS-IR > 实验海洋生物学重点实验室

	A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts
	Fan, Bolin 1; Chen, Funuan 1; Li, Yuan 1; Wang, Zhongliang 1; Wang, Zhiwen 1; Lu, Yishan 1,2; Wu, Zaohe 1; Jian, Jichang 1,3,4; Wang, Bei1,3,4
	2019-06-01
发表期刊	MOLECULAR OMICS
卷号	15 期号:3 页码:233-246
通讯作者	Wang, Bei([email protected])
摘要	The pathogenesis of tilapia meningoencephalitis is still unclear, where the involvement of circRNA is considered for its active role as a `` miRNA sponge''. Therefore, we aimed to investigate the profile of circRNA in tilapia meningoencephalitis further by constructing the circRNA-miRNA network for in-depth mechanism exploration. Briefly, a nile tilapia model of meningitis was established by injecting Streptococcus agalactiae (1.0 x 10(7) cfu per mL) and we evaluated the infected tilapia brain for the expression profile of circRNAs, their potential functions and their correlation with genes involved in inflammatory pathways. A total of 11 263 circRNAs were identified from RNA sequencing (RNA-seq) data in nile tilapia (Oreochromis niloticus), a commercially important fish in China and East Asia. GO and KEGG analyses revealed that the biological functions of genes hosting the circRNAs were enriched in the progression of metabolism and binding. Notably, we found that 99% circRNAs in tilapia had abundant miRNA-binding sites and a total of 2136 of the identified circRNAs had two to six miRNA-binding sites. Six circRNAs were validated by qRT-PCR and the final circRNA-miRNA network was constructed. This is the first report of comprehensive identification of O. niloticus circRNAs being differentially regulated in the brain in normal conditions relating to S. agalactiae infection. This work will shed novel light on gene expression mechanisms under disease conditions and may identify circRNAs as new biomarkers for meningoencephalitis and neurodegenerative disorders.
DOI	10.1039/c9mo00025a
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31702386]###2804; International Cooperation Science & Technology Planning Project of Guangdong Province of China[2017A050501037]###2805; National Natural Science Foundation of China[31702386]; International Cooperation Science & Technology Planning Project of Guangdong Province of China[2017A050501037]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000474156900004
出版者	ROYAL SOC CHEMISTRY
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161930
专题	实验海洋生物学重点实验室
通讯作者	Wang, Bei
作者单位	1.Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim Guangdong Higher E, Coll Fishery, Zhanjiang, GD, Peoples R China 2.Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, GD, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Fan, Bolin,Chen, Funuan,Li, Yuan,et al. A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts[J]. MOLECULAR OMICS,2019,15(3):233-246.
APA	Fan, Bolin.,Chen, Funuan.,Li, Yuan.,Wang, Zhongliang.,Wang, Zhiwen.,...&Wang, Bei.(2019).A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts.MOLECULAR OMICS,15(3),233-246.
MLA	Fan, Bolin,et al."A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts".MOLECULAR OMICS 15.3(2019):233-246.

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