Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein

doi:10.3390/molecules24101946

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	Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein
	Wang, Xueqin 1,2; Yu, Huahua1,2 ; Xing, Ronge1,2 ; Liu, Song 1,2; Chen, Xiaolin 1,2; Li, Pengcheng1,2
	2019-05-02
发表期刊	MOLECULES
ISSN	1420-3049
卷号	24 期号:10 页码:17
通讯作者	Li, Pengcheng([email protected])
摘要	The aim of this study was to isolate and purify antioxidative peptides from Pacific herring (Clupea pallasii) protein. Five enzymes (pepsin, trypsin, papain, flavourzyme, and neutrase) were used for protein hydrolysis, and Pacific herring protein hydrolysates (PHPH) were separated by ultrafiltration. The fraction with the molecular weight below 3500 Da exhibited the highest in vitro antioxidant activities and cellular antioxidant activity. The PHPH was isolated and purified by consecutive chromatographic methods including gel filtration chromatography and reverse high-performance liquid chromatography (RP-HPLC). The purified antioxidant peptides were identified as Leu-His-Asp-Glu-Leu-Thr (MW = 726.35 Da) and Lys-Glu-Glu-Lys-Phe-Glu (MW = 808.40 Da), and the IC50 values of cellular antioxidant activity were 1.19 +/- 0.05 mg/mL and 1.04 +/- 0.06 mg/mL. The results demonstrate that is possible to produce natural antioxidative peptides from Pacific herring protein via enzymatic hydrolysis and purification.
关键词	pacific herring protein hydrolysate peptides antioxidant activity purification
DOI	10.3390/molecules24101946
收录类别	SCI
语种	英语
资助项目	Key Research and Development Program of Shandong Province[2017YYSP018]###820; STS Project of the Chinese Academy of Sciences[2017T3006]###2768; Qingdao Science and Technology Project[17-3-3-21-nsh]###213; Key Research and Development Program of Shandong Province[2017YYSP018]; STS Project of the Chinese Academy of Sciences[2017T3006]; Qingdao Science and Technology Project[17-3-3-21-nsh]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000470996600104
出版者	MDPI
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161784
专题	实验海洋生物学重点实验室
通讯作者	Li, Pengcheng
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein[J]. MOLECULES,2019,24(10):17.
APA	Wang, Xueqin,Yu, Huahua,Xing, Ronge,Liu, Song,Chen, Xiaolin,&Li, Pengcheng.(2019).Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein.MOLECULES,24(10),17.
MLA	Wang, Xueqin,et al."Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein".MOLECULES 24.10(2019):17.

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