Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Community Structure of Bacteria Associated With Drifting Sargassum horneri, the Causative Species of Golden Tide in the Yellow Sea | |
Mei, Xiangyuan1,2,3,4; Wu, Chunhui1,2,3 ; Zhao, Jin1,2,3; Yan, Tian3,5,6; Jiang, Peng1,2,3
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| 2019-05-28 | |
| 发表期刊 | FRONTIERS IN MICROBIOLOGY
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| ISSN | 1664-302X |
| 卷号 | 10页码:17 |
| 通讯作者 | Jiang, Peng([email protected]) |
| 摘要 | Golden tides dominated by Sargassum spp. are occurring at an accelerated rate worldwide. In China, Sargassum has started to bloom in the Yellow Sea and led to tremendous economic losses, but the underlying biological causes and mechanisms are still unclear. Although algae-associated bacteria were suggested to play crucial roles in algal blooms, the profiles of bacteria associated with drifting Sargassum remain unexplored. In this study, the community structures and functions of Sargassum-associated bacteria were analyzed using the high-throughput sequencing data of the V5-V7 hypervariable region of the 16S rRNA gene. Molecular identification revealed that the golden tide analyzed in the Yellow Sea was dominated by a single species, Sargassum horneri. They were a healthy brown color nearshore but were yellow offshore with significantly decreased chlorophyll contents (P < 0.01), which indicates that yellow S. horneri was under physiological stress. The structural and functional analyses of bacterial communities indicated that the drifting S. horneri had an obvious selectivity on their associated bacteria against surrounding seawater. Although the bacterial communities phylogenetically differed between brown and yellow S. horneri (P < 0.01), their dominant functions were all nitrogen and iron transporters, which strongly indicates microbial contribution to blooming of the algal host. For the first time, potential epiphytic and endophytic bacteria associated with Sargassum were independently analyzed by a modified co-vortex method with silica sand. We showed that the composition of dominant endophytes, mainly Bacillus and Propionibacterium, was relatively consistent regardless of host status, whereas the epiphytic operational taxonomic units (OTUs) greatly varied in response to weakness of host status; however, dominant functions were consistent at elevated intensities, which might protect the host from stress related to nitrogen or iron deficiency. Thus, we propose that host physiological status at different intensities of functional demands, which were related to variable environmental conditions, may be a critical factor that influences the assembly of epiphytic bacterial communities. This study provided new insight into the structure and potential functions of associated bacteria with golden tide blooms. |
| 关键词 | 16S rRNA gene high-throughput sequencing bacterial community golden tide Sargassum horneri the Yellow Sea |
| DOI | 10.3389/fmicb.2019.01192 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050302]###2658; National Natural Science Foundation of China[41776153]###793; Open Fund of Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES201801]###2659; Open Fund of Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0303]###2660; Marine S&T Fund of Shandong Province[2018SDKJ050404]###2661; Nantong Municipal Science and Technology Project[MS12017019-4]###2646; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050302]; National Natural Science Foundation of China[41776153]; Open Fund of Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES201801]; Open Fund of Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0303]; Marine S&T Fund of Shandong Province[2018SDKJ050404]; Nantong Municipal Science and Technology Project[MS12017019-4] |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| WOS记录号 | WOS:000469262300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/161563 |
| 专题 | 实验海洋生物学重点实验室 海洋生态与环境科学重点实验室 |
| 通讯作者 | Jiang, Peng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Mei, Xiangyuan,Wu, Chunhui,Zhao, Jin,et al. Community Structure of Bacteria Associated With Drifting Sargassum horneri, the Causative Species of Golden Tide in the Yellow Sea[J]. FRONTIERS IN MICROBIOLOGY,2019,10:17. |
| APA | Mei, Xiangyuan,Wu, Chunhui,Zhao, Jin,Yan, Tian,&Jiang, Peng.(2019).Community Structure of Bacteria Associated With Drifting Sargassum horneri, the Causative Species of Golden Tide in the Yellow Sea.FRONTIERS IN MICROBIOLOGY,10,17. |
| MLA | Mei, Xiangyuan,et al."Community Structure of Bacteria Associated With Drifting Sargassum horneri, the Causative Species of Golden Tide in the Yellow Sea".FRONTIERS IN MICROBIOLOGY 10(2019):17. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Community Structure,（7301KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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