An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP

doi:10.1016/j.dci.2019.03.014

IOCAS-IR > 实验海洋生物学重点实验室

	An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP
	Sun, Mingzhe1,2,3 ; Li, Shihao 1,2,4; Yu, Kuijie 1,2,4; Xiang, Jianhai1,2 ; Li, Fuhua 1,2,4
	2019-08-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	97 页码:57-63
通讯作者	Li, Fuhua([email protected])
摘要	The TRIpartite Motif (TRIM) proteins are known to play key roles in cell differentiation, apoptosis, development, autophagy and innate immunity. In the present study, a TRIM9 homolog (named LvTRIM9) was identified from the transcriptome of the Pacific whiteleg shrimp Litopenaeus vannamei. The deduced amino acid sequence of LvTRIM9 possessed typical features of TRIMs, consisting of a RING domain, two B-boxes, a coiled-coil domain, a FN3 domain, and a SPRY domain. The transcript of LvTRIM9 was detected in most tissues of the shrimp. Its expression level was obviously up-regulated at 3, 12 and 24 h post white spot syndrome virus (WSSV) infection. Knockdown of LvTRIM9 gene expression by double-strand RNA mediated interference could lead to a decrease of virus copy number in WSSV-infected shrimp. Yeast two-hybrid analysis showed that LvTRIM9 could directly interact with beta-transducin repeat-containing protein of shrimp (Lv beta-TrCP), an inhibitor of NF-kappa B pathway. Meanwhile, knockdown of LvTRIM9 could also up-regulate the expression levels of LvRelish and downstream production of antimicrobial peptides in the intestine of shrimp. These data indicated that WSSV might hijack the LvTRIM9 for its propagation through inhibition of NF-kappa B pathway and downstream antimicrobial peptides production via interaction of LvTRIM9 with Lv beta-TrCP in shrimp. The study improved our understanding about the impact of E3 ubiquitin ligases on the innate immune signaling pathway of shrimp and its role during WSSV infection.
关键词	TRIM9 beta-TrCP White spot syndrome virus NB-kappa B pathway Antimicrobial peptides
DOI	10.1016/j.dci.2019.03.014
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0900502]###2645; China Agriculture Research system-48 (CARS-48)###148; National Natural Science Foundation of China[41776158]###147; National Natural Science Foundation of China[31772880]###146; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04]###145; National Key Research and Development Program of China[2018YFD0900502]; China Agriculture Research System-48 (CARS-48); National Natural Science Foundation of China[41776158]; National Natural Science Foundation of China[31772880]; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04]
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000468721600007
出版者	ELSEVIER SCI LTD
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161425
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Mingzhe,Li, Shihao,Yu, Kuijie,et al. An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,97:57-63.
APA	Sun, Mingzhe,Li, Shihao,Yu, Kuijie,Xiang, Jianhai,&Li, Fuhua.(2019).An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,97,57-63.
MLA	Sun, Mingzhe,et al."An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 97(2019):57-63.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S0145305X1930（2687KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览
An E3 ubiquitin liga（2687KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览