Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30

doi:10.3390/md17040199

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30
	Wu, Shimei 1; Liu, Ge2,3,4 ; Zhou, Shengnan 1; Sha, Zhenxia 1; Sun, Chaomin 2,3,4
	2019-04-01
发表期刊	MARINE DRUGS
ISSN	1660-3397
卷号	17 期号:4 页码:16
通讯作者	Sha, Zhenxia([email protected]) ; Sun, Chaomin([email protected])
摘要	This study was initiated to screen for marine bacterial agents to biocontrol Magnaporthe grisea, a serious fungal pathogen of cereal crops. A bacterial strain, isolated from the cold seep in deep sea, exhibited strong growth inhibition against M. grisea, and the strain was identified and designated as Bacillus sp. CS30. The corresponding antifungal agents were purified by acidic precipitation, sequential methanol extraction, Sephadex LH-20 chromatography, and reversed phase high-performance liquid chromatography (RP-HPLC), and two antifungal peaks were obtained at the final purification step. After analysis by mass spectrometry (MS) and tandem MS, two purified antifungal agents were deduced to belong to the surfactin family, and designated as surfactin CS30-1 and surfactin CS30-2. Further investigation showed that although the antifungal activity of surfactin CS30-1 is higher than that of surfactin CS30-2, both of them induced the increased generation of reactive oxygen species (ROS) and caused serious damage to the cell wall and cytoplasm, thus leading to the cell death of M. grisea. Our results also show the differences of the antifungal activity and antifungal mechanism of the different surfactin homologs surfactin CS30-1 and surfactin CS30-2, and highlight them as potential promising agents to biocontrol plant diseases caused by M. grisea.
关键词	lipopeptide surfactin Bacillus antifungal reactive oxygen species
DOI	10.3390/md17040199
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFC0310800]###373; China Ocean Mineral Resources RD Association[DY135-B2-14]###2443; Natural Science Outstanding Youth Fund of Shandong Province[JQ201607]###1593; Tai Shan Young Scholar Foundation of Shandong Province[tsqn20161051]###2444; AoShan Talents Program by Qingdao National Laboratory for Marine Science and Technology[2015ASTP]###1762; 100-Talent Project of the Chinese Academy of Sciences###1594; National Key R&D Program of China[2018YFC0310800]; China Ocean Mineral Resources RD Association[DY135-B2-14]; Natural Science Outstanding Youth Fund of Shandong Province[JQ201607]; Tai Shan Young Scholar Foundation of Shandong Province[tsqn20161051]; AoShan Talents Program by Qingdao National Laboratory for Marine Science and Technology[2015ASTP]; 100-Talent Project of the Chinese Academy of Sciences
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal
WOS记录号	WOS:000467307100006
出版者	MDPI
引用统计	被引频次：47[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161326
专题	实验海洋生物学重点实验室
通讯作者	Sha, Zhenxia; Sun, Chaomin
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wu, Shimei,Liu, Ge,Zhou, Shengnan,et al. Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30[J]. MARINE DRUGS,2019,17(4):16.
APA	Wu, Shimei,Liu, Ge,Zhou, Shengnan,Sha, Zhenxia,&Sun, Chaomin.(2019).Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30.MARINE DRUGS,17(4),16.
MLA	Wu, Shimei,et al."Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30".MARINE DRUGS 17.4(2019):16.

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