Layered double hydroxide derived ultrathin 2D Ni-V mixed metal oxide as a robust peroxidase mimic

doi:10.1016/j.cej.2019.03.070

	Layered double hydroxide derived ultrathin 2D Ni-V mixed metal oxide as a robust peroxidase mimic
	Chen, Chao1,2,3,4 ; Wang, Yi1,3,4 ; Yang, Zhiqing 1,2,3,4; Zhang, Dun 1,3,4
	2019-08-01
发表期刊	CHEMICAL ENGINEERING JOURNAL
ISSN	1385-8947
卷号	369 页码:161-169
通讯作者	Wang, Yi([email protected]) ; Zhang, Dun([email protected])
摘要	Herein, we first show that layered double hydroxide derived ultrathin 2D Ni-V mixed metal oxide (MMO) nanosheets with thickness of sub-10 nm exhibit an robust peroxidase (POx) mimic activity, accelerating H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce a color reaction. The effect of varying the Ni/V molar ratio and heating temperature on the POx mimic activity and physicochemical properties of Ni-V MMOs has also been investigated in detail. Ni-V MMO is composed of NiO and Ni3V2O8 and under optimized conditions, Ni3V2O8 nanoparticles with particle size of sub-5 nm are well dispersed on the 2D NiO nanosheet matrix. Ni-V MMO with a Ni/V molar ratio of 3:1 calcinated at 400 degrees C shows best POx mimic activity. The catalytic mechanism was analyzed by electron spin resonance spectroscopy, which suggested that the enzyme mimic activities of Ni-V MMO originated from the decomposition of H2O2 to generate reactive (OH)-O-center dot radicals. Kinetic analysis indicates typical Michaelis-Menten catalytic behavior. The findings were used to design a colorimetric assay for H2O2, best measured at 652 nm. The method has a linear response in the 10-100 mM H2O2 concentration range, with a 5.7 mM detection limit. Benefitting from the high dispersibility and high specific surface area of the ultrathin 2D nanosheets, the method is well reproducible. It's also easily performed and highly specific. The current work can provide new strategies to construct novel LDH derived nanoenzymes for biosensor, biotechnology, and biomedicine.
关键词	Layered double hydroxide Mixed metal oxide Nanosheet Enzyme mimic Kinetic Colorimetric detection
DOI	10.1016/j.cej.2019.03.070
收录类别	SCI
语种	英语
资助项目	Natured Science Foundation of China[41776090]###56; Natured Science Foundation of China[41476068]###57; National Key Research and Development Program of China[2016YFC1400605]###58; Key Research and Development Program of Shandong Province[2018GHY115038]###677; AoShan Talent Program###510; Qingdao National Laboratory for Marine Science and Technology###117; Natured Science Foundation of China[41776090]; Natured Science Foundation of China[41476068]; National Key Research and Development Program of China[2016YFC1400605]; Key Research and Development Program of Shandong Province[2018GHY115038]; AoShan Talent Program; Qingdao National Laboratory for Marine Science and Technology
WOS研究方向	Engineering
WOS类目	Engineering, Environmental ; Engineering, Chemical
WOS记录号	WOS:000463344800017
出版者	ELSEVIER SCIENCE SA
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161174
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Wang, Yi; Zhang, Dun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chen, Chao,Wang, Yi,Yang, Zhiqing,et al. Layered double hydroxide derived ultrathin 2D Ni-V mixed metal oxide as a robust peroxidase mimic[J]. CHEMICAL ENGINEERING JOURNAL,2019,369:161-169.
APA	Chen, Chao,Wang, Yi,Yang, Zhiqing,&Zhang, Dun.(2019).Layered double hydroxide derived ultrathin 2D Ni-V mixed metal oxide as a robust peroxidase mimic.CHEMICAL ENGINEERING JOURNAL,369,161-169.
MLA	Chen, Chao,et al."Layered double hydroxide derived ultrathin 2D Ni-V mixed metal oxide as a robust peroxidase mimic".CHEMICAL ENGINEERING JOURNAL 369(2019):161-169.

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