Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel

doi:10.1007/s00343-019-7400-1

	Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel
	Li Xiaohong 1,2,3; Xiao Hui 1; Zhang Wenjun 4; Li Yongqian 1,2,3; Tang Xuexi 1; Duan Jizhou2,3 ; Yang Zhibo 1; Wang Jing1,2,3 ; Guan Fang2,3 ; Ding Guoqing 5
	2019-03-01
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
卷号	37 期号:2 页码:600-614
通讯作者	Tang Xuexi([email protected]) ; Duan Jizhou([email protected])
摘要	Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited attention has been paid to the composition and function of aerobic and facultative bacteria in this process. For analysis in this study, ten samples were collected from rust layers on steel plates that had been immersed in seawater for different periods (i.e., six months and eight years) at Sanya and Xiamen, China. The cultivable aerobic bacterial community structure as well as the number of sulfate-reducing bacteria (SRB) were analyzed in both cases, while the proportion of facultative SRB among the isolated aerobic bacteria in each sample was also evaluated using a novel approach. Bacterial abundance results show that the proportions are related to sea location and immersion time; abundances of culturable aerobic bacteria (CAB) and SRB from Sanya were greater in most corrosion samples than those from Xiamen, and abundances of both bacterial groups were greater in samples immersed for six months than for eight years. A total of 213 isolates were obtained from all samples in terms of CAB community composition, and a phylogenetic analysis revealed that the taxa comprised four phyla and 31 genera. Bacterial species composition is related to marine location; the results show that Firmicutes and Proteobacteria were the dominant phyla, accounting for 98.13% of the total, while Bacillus and Vibrio were the dominant genera, accounting for 53.06% of the total. An additional six facultative SRB strains were also screened from the isolates obtained and were found to encompass the genus Vibrio (four strains), Staphylococcus (one strain), and Photobacterium (one strain). It is noteworthy that mentions of Photobacterium species have so far been absent from the literature, both in terms of its membership of the SRB group and its relationship to corrosion.
关键词	marine corrosive steel cultivable aerobic bacteria facultative sulfate-reducing bacteria bacterial community composition 16S rRNA gene sequencing
DOI	10.1007/s00343-019-7400-1
收录类别	SCI
语种	英语
资助项目	National Basic Research Program of China (973 Program)[2014CB643304]###2549; National Natural Science Foundation of China[41576080]###2144; Key Research and Development Program of Shandong Province[2018GHY115003]###2550; National Basic Research Program of China (973 Program)[2014CB643304]; National Natural Science Foundation of China[41576080]; Key Research and Development Program of Shandong Province[2018GHY115003]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000464895600019
出版者	SCIENCE PRESS
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161162
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Tang Xuexi; Duan Jizhou
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, Qingdao 266235, Shandong, Peoples R China 4.Ningbo Tianhe Aquat Ecosyst Engn Co Ltd, Ningbo 315000, Zhejiang, Peoples R China 5.Qingdao Res Inst Marine Corros, Qingdao 266000, Shandong, Peoples R China
第一作者单位	海洋环境腐蚀与生物污损重点实验室
通讯作者单位	海洋环境腐蚀与生物污损重点实验室
推荐引用方式 GB/T 7714	Li Xiaohong,Xiao Hui,Zhang Wenjun,et al. Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(2):600-614.
APA	Li Xiaohong.,Xiao Hui.,Zhang Wenjun.,Li Yongqian.,Tang Xuexi.,...&Ding Guoqing.(2019).Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(2),600-614.
MLA	Li Xiaohong,et al."Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.2(2019):600-614.

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