Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure

doi:10.1016/j.cbpc.2018.12.003

IOCAS-IR

	Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure
	Dong, Shihang 1; Yang, Yanyan 2; Cheng, Bo 3; Ren, Chuanbo 2; Zhang, Huawei 2; Xu, Hua 4; Zhu, Na 1; Wang, Wansheng 1; Dang, Yongjian 1; Li, Mingzhu 1; Chen, Jun 1; Wang, Kai1 ; Zhang, Libin 5; Fang, Yan 1
	2019-03-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN	1532-0456
卷号	217 页码:98-105
通讯作者	Fang, Yan([email protected])
摘要	Antioxidant enzymes play essential roles against oxidative stress caused by 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), which is ubiquitous in marine environment and organisms. However, research on antioxidant responses to BDE-47 in marine bivalves is scarce. In this study, we identified the full-length cDNA of catalase (CAT), and glutathione peroxidase (GPx) in the clam Mactra veneriformis. Subsequently, the responses of CAT, GPx, and copper, zinc-superoxide dismutase (Cu, Zn-SOD) were investigated in the clams exposed to 0.1, 1, and 10 mu g/L BDE-47 for 7 days, and then depurated in natural seawater for 3 days. MvCAT and MvGPx contained conserved sequences. The deduced amino acid sequences shared high similarity with CATs and GPxs in other mollusks. M. veneriformis accumulated BDE-47 in a dose-dependent manner and eliminated BDE-47 poorly. BDE-47 induced a time- and dose-dependent increase of malondialdehyde content. Both the dose and the duration had significant effect on mRNA expressions and activities of the three antioxidants. Cu, Zn-SOD responded to BDE-47 earlier than CAT and GPx. The antioxidant responses could recover after depuration. These results suggested that M. veneriformis could accumulate BDE-47 efficiently. Antioxidant enzymes were triggered to counter the oxidative stress generated by BDE-47. Cu, Zn-SOD acted as the first defense against oxidative stress, while CAT and GPx intervened later. This study is therefore helpful in understanding the antioxidant responses to PBDEs in marine bivalves.
关键词	Catalase Glutathione peroxidase Superoxide dismutase 2,2 ',4,4 '-Tetrabromodiphenyl ether Malondialdehyde Mactra veneriformis
DOI	10.1016/j.cbpc.2018.12.003
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41206120]###2529; National Natural Science Foundation of China[31502191]###2530; National Natural Science Foundation of China[31502173]###2531; Development Plan of Science and Technology in Yantai[2011062]###2532; Ludong University Research Funding[210-32040301]###2533; Innovative Project of Undergraduate[201710451328]###2534; Innovative Project of Undergraduate[171133]###2535; National Natural Science Foundation of China[41206120]; National Natural Science Foundation of China[31502191]; National Natural Science Foundation of China[31502173]; Development Plan of Science and Technology in Yantai[2011062]; Ludong University Research Funding[210-32040301]; Innovative Project of Undergraduate[201710451328]; Innovative Project of Undergraduate[171133]
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS记录号	WOS:000463848100011
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161147
专题	中国科学院海洋研究所
通讯作者	Fang, Yan
作者单位	1.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China 2.Shandong Marine Resource & Environm Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China 3.Chinese Acad Fishery Sci, Aquat Prod Qual & Stand Res Ctr, Beijing 100141, Peoples R China 4.Yantai Environm Monitoring Ctr Stn, Yantai 264000, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Dong, Shihang,Yang, Yanyan,Cheng, Bo,et al. Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2019,217:98-105.
APA	Dong, Shihang.,Yang, Yanyan.,Cheng, Bo.,Ren, Chuanbo.,Zhang, Huawei.,...&Fang, Yan.(2019).Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,217,98-105.
MLA	Dong, Shihang,et al."Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 217(2019):98-105.