Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas | |
Wang, Xiudan1 ; Zhao, Xiaoli1; Yan, Chunyu1; Jia, Zhihao2,3; Lv, Zhao2,4; Ma, Cuiping1; Wang, Mengqiang2,5,6,7
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| 2019-05-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 88页码:318-327 |
| 通讯作者 | Wang, Mengqiang([email protected]) |
| 摘要 | A variety of combinations of leucine-rich repeat (LRR) and immunoglobulin-like (Ig) domains have been found and discovered in invertebrates and vertebrates, but the functions remain largely unexplored. In the present study, a novel LRR and Ig domain-containing protein (LRRIG), CgLRRIG-3, was identified and characterized from oyster Crassostrea gigas. It contained two typical LRR motifs, a LRRNT motif and an Ig domain and PSI-BALST and phylogeny analysis revealed that the sequence of CgLRRIG-3 was most related with leucine-rich repeat neuronal 1 proteins from vertebrate. Its mRNA transcripts were constitutively expressed in muscle, gill, hepatopancreas, mantle, gonad and hemocytes with the highest level in hepatopancreas. The mRNA expression level of CgLRRIG-3 in hemocytes could respond to the stimulations of variety PAMPs including lipopolysaccharide (LPS), peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C). The recombinant proteins exhibited a wide PAMP binding repertoire to four typical PAMPs and could significantly induce the expression of CgTNF-1 and CgIL17-5 as well as increase phagocytosis in primary cultured oyster hemocytes. In hepatopancreas, CgLRRIG-3 was mainly distributed in the basolateral membrane of digestive tubule and the hemocoel sinusoid between the digestive tubules. And in hemocytes, the positive signal was mainly distributed in a special group of granulocytes. These results collectively indicated that CgLRRIG-3 could not only function as an immune effector. |
| 关键词 | Crassostrea gigas Leucine-rich repeat domain Immunoglobulin domain LRRIG Pattern recognition receptor Immune effector |
| DOI | 10.1016/j.fsi.2019.03.003 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31800414]###2499; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2018N01]###2500; Research Initial Funding Project for Doctors in Qingdao University of Science and Technology[010022908]###2501; National Natural Science Foundation of China[31800414]; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2018N01]; Research Initial Funding Project for Doctors in Qingdao University of Science and Technology[010022908] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000465060500038 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/161105 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Mengqiang |
| 作者单位 | 1.Qingdao Univ Sci & Technol, Shandong Prov Key Lab Biochem Engn, Coll Marine Sci & Biol Engn, Qingdao 266042, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China 6.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 7.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Wang, Xiudan,Zhao, Xiaoli,Yan, Chunyu,et al. A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:318-327. |
| APA | Wang, Xiudan.,Zhao, Xiaoli.,Yan, Chunyu.,Jia, Zhihao.,Lv, Zhao.,...&Wang, Mengqiang.(2019).A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,88,318-327. |
| MLA | Wang, Xiudan,et al."A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 88(2019):318-327. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A novel LRR and Ig d（2872KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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