Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research

doi:10.1080/09670262.2018.1536283

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research
	Li, Qianqian1 ; Zhang, Litao 1,2; Pang, Tong 1,2; Liu, Jianguo 1,2
	2019-04-03
发表期刊	EUROPEAN JOURNAL OF PHYCOLOGY
ISSN	0967-0262
卷号	54 期号:2 页码:162-174
通讯作者	Liu, Jianguo([email protected])
摘要	Kappaphycus alvarezii provides an important raw material for the extraction of kappa-carrageenan. Comparative transcriptome analysis of K. alvarezii after 24 h extreme temperature treatments (17 degrees C and 34 degrees C) was performed to uncover the molecular mechanisms controlling temperature-related photosynthesis regulation. Optimum temperature (28 degrees C) was employed as the control. After de novo assembly of these three transcriptome samples and searching the assembled transcripts by BLAST against the NCBI nr databases, 11,537 top-hit transcripts were assigned as unigenes. Through comparisons, 302 significantly differentially expressed genes were identified. Twenty-three of the differentially expressed unigenes encoded photosystem antenna protein subunits and a further 25 were associated more broadly with photosynthesis functions. Compared to the light-harvesting complex (LHC) protein, the expression of phycobiliproteins (PBPs) was stable after extreme low and high temperature (ELT/EHT) treatments. Both ELT and EHT were found to inhibit the expression of subunits of photosystem II (PsbA, PsbC, Psb27, PsbW, PsbS), photosystem I (PsaF, PsaG, PsaK) and photosynthetic electron transport (PetE). These results were confirmed through targeted analysis of the mRNA and protein abundance of individual proteins, and were congruent with the results of chlorophyll a fluorescence (OJIP) measurements performed with temperature-stressed lines. In conclusion, similarities and differences in the behaviours of photosynthesis-related genes were summarized to provide opportunities for understanding the responses of K. alvarezii after extreme temperature treatments.
关键词	Antenna protein extreme temperature Kappaphycus alvarezii photosynthesis thylakoid membrane proteins transcriptome
DOI	10.1080/09670262.2018.1536283
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31702366]###432; National Natural Science Foundation of China[41306154]###2496; Open Foundation of Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences[LMB17011007]###2497; National Natural Science Foundation of China[31702366]; National Natural Science Foundation of China[41306154]; Open Foundation of Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences[LMB17011007]
WOS研究方向	Plant Sciences ; Marine & Freshwater Biology
WOS类目	Plant Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000463785700003
出版者	TAYLOR & FRANCIS LTD
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161093
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Qianqian,Zhang, Litao,Pang, Tong,et al. Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2019,54(2):162-174.
APA	Li, Qianqian,Zhang, Litao,Pang, Tong,&Liu, Jianguo.(2019).Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research.EUROPEAN JOURNAL OF PHYCOLOGY,54(2),162-174.
MLA	Li, Qianqian,et al."Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research".EUROPEAN JOURNAL OF PHYCOLOGY 54.2(2019):162-174.

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