Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus

doi:10.1016/j.fsi.2018.11.005

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus
	Ning, Junhao 1,3,4; Liu, Yuan 1,2,3; Gao, Fengtao 1,2,3; Liu, Hourong 1,3,4; Cui, Zhaoxia 1,2,3
	2019
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	84 页码:970-978
通讯作者	Liu, Yuan([email protected]) ; Cui, Zhaoxia([email protected])
摘要	The receptor for the globular head of complement component C1q, gC1qR, is a multifunctional and multiligand binding protein with a crucial role in host defense. In the present study, a full-length cDNA sequence of a gC1qR homolog (PtgC1qR) in Portunus trituberculoms was identified. PtgC1qR was a 268-amino-acid polypeptide with a conserved MAM33 domain and a mitochondrial targeting sequence in the first 56 amino acids. The transcripts of PtgC1qR were detected in all examined tissues with the highest level detected in the hepatopancreas. Compared with other early embryonic stages, PtgC1qR was highly expressed in the fertilized eggs and embryos at the cleavage stage, which suggest PtgC1qR may be a maternal gene. The transcripts of PtgC1qR in hemocytes exhibited time-dependent response expression pattern after challenged with bacteria (Vibrio alginolyticus, Micrococcus luteus) and fungi (Pichia pastoris). Moreover, the recombinant PtgC1qR (rPtgC1qR) exhibited strong antibacterial activity and microbial-binding activity, suggesting its crucial role in immune defense and recognition. Further phenoloxidase (PO) assay showed that rPtgC1qR could suppress the crab PO activity in vitro in a dose-dependent manner, and it could result in nearly 100% inhibition of PO activity under the concentration of 11.65 mu M. Knockdown of PtgC1qR could significantly enhance the expression of serine protease related genes (PtSP1-3 and PISPH), proPO-associated genes (PiproPO and PtPPAF) and C3-like genes (Pta2M1 and PtTEP). However, the phagocytosis related genes (PtMyosin, PtRab5 and PtArp) and Pta2M2 were significantly down-regulated in the PtgC1qR silenced crabs. These findings together demonstrate that PtgC1qR might function in crab immune response via its antibacterial activity, immune recognition or regulating the proPO system, complement pathway and phagocytosis.
关键词	Portunus trituberculatus gC1qR Expression profiles Antimicrobial activity Microbial binding activity Prophenoloxidase activating system
DOI	10.1016/j.fsi.2018.11.005
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41776159]###2453; National Natural Science Foundation of China[41206147]###2454; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]###1178; Natural Science Foundation of Shandong Province[ZR2017QD001]###2412; National Natural Science Foundation of China[41776159]; National Natural Science Foundation of China[41206147]; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]; Natural Science Foundation of Shandong Province[ZR2017QD001]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000457666800105
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/160868
专题	实验海洋生物学重点实验室
通讯作者	Liu, Yuan; Cui, Zhaoxia
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Ning, Junhao,Liu, Yuan,Gao, Fengtao,et al. Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:970-978.
APA	Ning, Junhao,Liu, Yuan,Gao, Fengtao,Liu, Hourong,&Cui, Zhaoxia.(2019).Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,84,970-978.
MLA	Ning, Junhao,et al."Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 84(2019):970-978.

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