Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22

doi:10.1016/j.fsi.2018.09.068

IOCAS-IR > 实验海洋生物学重点实验室

	Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22
	Li, Yi-qun 1,2,3,4; Sun, Li 1,2,3; Li, Jun 2,3,5
	2019
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	84 页码:138-147
通讯作者	Sun, Li([email protected]) ; Li, Jun([email protected])
摘要	B cells in fish are proven to be endocytic and have a great contribution to innate immunity like phagocytosis. In this study, the endocytic capacity and the corresponding internalization pathways of IgM(+) B cells in Japanese flounder (Paralichthys olivaceus) were investigated. The results showed that IgM(+) B cells in peripheral blood leukocytes (PBL) and splenic leukocytes (SL) exhibited different abilities to ingest 0.5 mu m and 1 pm latex beads through macropinocytosis-dependent endocytic pathway. Japanese flounder CD22 (PoCD22) co-stimulatory signals were identified to be essential for the innate immune responses in B cells. Most of IgM+ B cells and some IgM(-) cells were demonstrated to be PoCD22 positive. When PoCD22 was blocked by antibody, the endocytic activities and reactive oxygen species (ROS) activities of SL IgM(+) B cells were significantly increased, while the endocytic and ROS activities of PBL IgM(+) B cells were significant decreased. These results collectively suggest that Japanese flounder IgM(+) B cells are able to employ macropinocytosis-dependent endocytic pathway, which is under the regulation of CD22.
关键词	Paralichthys olivaceus IgM(+) B cells Endocytosis Macropinocytosis CD22 B cell regulation
DOI	10.1016/j.fsi.2018.09.068
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31528019]###1469; AoShan Talents Program from Qingdao National Laboratory for Marine Science and Technology[2015ASTP]###1470; Taishan Scholar Program of Shandong Province###423; National Natural Science Foundation of China[31528019]; AoShan Talents Program from Qingdao National Laboratory for Marine Science and Technology[2015ASTP]; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000457666800017
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/160826
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Lake Super State Univ, Sch Biol Sci, 650 W Easterday Ave, Sault Ste Marie, MI 49783 USA
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Yi-qun,Sun, Li,Li, Jun. Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:138-147.
APA	Li, Yi-qun,Sun, Li,&Li, Jun.(2019).Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22.FISH & SHELLFISH IMMUNOLOGY,84,138-147.
MLA	Li, Yi-qun,et al."Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22".FISH & SHELLFISH IMMUNOLOGY 84(2019):138-147.

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