Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi)

doi:10.1016/j.cbpb.2018.10.006

IOCAS-IR

	Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi)
	Dong, Junjian 1; Chen, Zhihang 3; Sun, Chengfei 1,2; Tian, Yuanyuan 1; Hu, Jie 1; Lu, Maixin 1,2; Ye, Xing 1,2
	2019-02-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	228 页码:10-16
通讯作者	Ye, Xing([email protected])
摘要	Myosin heavy chains (MYHs) play important roles in muscle growth and contraction. In fish, MYHs contribute to hyperplasia and hypertrophy of muscle fibers, which can continue into adult life and thus result in indeterminate growth in some species. We previously identified two MYH genes, MYH-7a and MYH-7b, that are differentially expressed in Mandarin fish (Siniperca chuatsi) and appear to function in early growth. However, the regulatory role of their 5' flanking regions is unknown. To examine the effects of single nucleotide polymorphisms (SNPs) in these regions, we used genome walking to amplify their flanking sequences and analyzed the regulatory elements and binding sites. A single SNP locus was found in the flanking sequence of each gene. These SNP loci are located in the conserved glucocorticoid receptor binding region (MYH-7a G-614A; Allele frequency: G:A = 94.9:5.1; GG (89.76) and AG (10.24) genotypes) and the LIM homeobox domain transcription factor binding sequence (MYH-76: C-1933A; Allele frequency: C:A = 54.8:45.2; AA (20.82), AC (48.81), and CC (30.37) genotypes). At the G-614A loci, the GG genotype exhibited more superior growth traits (total length, body length, body height, etc.) than the AG genotype, with the exception of caudal peduncle length. Alternatively, at the C-1933A loci, the AC and AA genotypes showed significant differences in all growth traits, except for head length, with AC exhibiting superior traits. The AA and CC genotypes showed significant differences in caudal peduncle length and height, while no differences were observed between the AC and CC genotypes. Thus, these SNPs in the 5' flanking regions of MYH-7a and MYH-7b are correlated with superior growth and can be used for selecting Mandarin fish during breeding.
关键词	5' flanking region Growth correlation analysis Mandarin fish (Siniperca chuatsi) Myosin heavy chain gene (MYH-7) Single nucleotide polymorphism (SNP)
DOI	10.1016/j.cbpb.2018.10.006
收录类别	SCI
语种	英语
资助项目	Central Public-interest Scientific Institution Basal Research Fund CAFS[2017HY-ZC0402]###2334; China Agriculture Research System[CARS-46]###2335; Ocean Fisheries Science and Technology Promotion Project of Guangdong Province[A201601A06]###2336; Science and Technology Project of Guangdong Province[2015A020209034]###2337; Central Public-interest Scientific Institution Basal Research Fund CAFS[2017HY-ZC0402]; China Agriculture Research System[CARS-46]; Ocean Fisheries Science and Technology Promotion Project of Guangdong Province[A201601A06]; Science and Technology Project of Guangdong Province[2015A020209034]
WOS研究方向	Biochemistry & Molecular Biology ; Zoology
WOS类目	Biochemistry & Molecular Biology ; Zoology
WOS记录号	WOS:000456639400002
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/160612
专题	中国科学院海洋研究所
通讯作者	Ye, Xing
作者单位	1.Chinese Acad Fishery Sci, Pearl River Fisheries Inst, Key Lab Trop & Subtrop Fisheries Resource Applica, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China 2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Dong, Junjian,Chen, Zhihang,Sun, Chengfei,et al. Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2019,228:10-16.
APA	Dong, Junjian.,Chen, Zhihang.,Sun, Chengfei.,Tian, Yuanyuan.,Hu, Jie.,...&Ye, Xing.(2019).Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,228,10-16.
MLA	Dong, Junjian,et al."Cloning, SNP detection, and growth correlation analysis of the 5 ' lanking regions of two myosin heavy chain-7 genes in Mandarin fish (Siniperca chuatsi)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 228(2019):10-16.