Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge

doi:10.1016/j.fsi.2018.08.046

IOCAS-IR > 实验海洋生物学重点实验室

	Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge
	Zhang, Jiquan 1,2,3; Liu, Yujie 1; Li, Yanyan 1; Su, Naike 1; Zhou, Yaru 1; Xiang, Jianhai2,3 ; Sun, Yuying 1
	2018-11-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	82 页码:378-385
通讯作者	Sun, Yuying([email protected])
摘要	The gC1qR is a ubiquitously expressed cell protein that interacts with the globular heads of C1q (gC1q) and many other ligands. In this study, one gC1qR homolog gene was obtained from Exopalaemon carinicauda and named EcgC1qR. The complete nucleotide sequence of EcgC1qR contained a 774 bp open reading frame (ORF) encoding EcgC1qR precursor of 257 amino acids. The deduced amino acid sequence of EcgC1qR revealed a 55- amino-acid-long mitochondrial targeting sequence at the N-terminal and a mitochondrial acidic matrix protein of 33 kDa (MAM33) domain. The genomic organization of EcgC1qR gene showed that EcgC1qR gene contained five exons and four introns. EcgC1qR could express in all of the detected tissues and its expression was much higher in hepatopancreas and hemocytes. The expression of EcgC1qR in the hepatopancreas of prawns challenged with Vibrio parahaemolyticus and Aeromonas hydrophila changed in a time-dependent manner. The expression of EcgC1qR in prawns challenged with V. parahaemolyticus was up-regulated at 6h (p < 0.05), and significantly up-regulated at 12 h and 24 h (p < 0.01), and then returned to the control levels at 48 h post-challenge (p > 0.05). At the same time, the expression in Aeromonas-challenged group was significantly up-regulated at 6, 12 and 24 h. The recombinant EcgC1qR could inhibit the growth of two tested bacteria. In addition, we successfully deleted EcgC1qR gene through CRISPR/Cas9 technology and it was the first time to obtain the mutant of gC1qR homolog gene in crustacean. It's a great progress to study the biological function of gC1qR in crustacean in future.
关键词	Exopalaemon carinicauda Globular heads of C1q CRISPR/Cas9 Knock-out
DOI	10.1016/j.fsi.2018.08.046
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31872613]###1035; National Natural Science Foundation of China[41876196]###1036; National Natural Science Foundation of China[31772885]###1037; National Natural Science Foundation of China[31872613]; National Natural Science Foundation of China[41876196]; National Natural Science Foundation of China[31772885]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000447571600044
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/160437
专题	实验海洋生物学重点实验室
通讯作者	Sun, Yuying
作者单位	1.Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Jiquan,Liu, Yujie,Li, Yanyan,et al. Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2018,82:378-385.
APA	Zhang, Jiquan.,Liu, Yujie.,Li, Yanyan.,Su, Naike.,Zhou, Yaru.,...&Sun, Yuying.(2018).Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge.FISH & SHELLFISH IMMUNOLOGY,82,378-385.
MLA	Zhang, Jiquan,et al."Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge".FISH & SHELLFISH IMMUNOLOGY 82(2018):378-385.

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