Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri | |
Wang, M. Q.1,3; Wang, B. J.1; Liu, M.1; Jiang, K. Y.1; Wang, L.1,2,4
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| 2018 | |
| 发表期刊 | ISJ-INVERTEBRATE SURVIVAL JOURNAL
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| ISSN | 1824-307X |
| 卷号 | 15页码:294-301 |
| 通讯作者 | Wang, L.([email protected]) |
| 摘要 | The defender against apoptotic cell death 1 (DAD1) was a negative regulator to inhibit the apoptosis process. In the present study, a DAD1 gene (designated as CfDAD1) was identified in Zhikong scallop Chlamys farreri. Its full-length cDNA sequence of CfDAD1 contained a 342 bp open reading frame (ORF), which encoded a mature protein of 113 amino acids. A DAD domain was revealed from the deduced protein sequence of CfDAD1. The mRNA transcripts of CfDAD1 could be detectable in all the investigated tissues, including hemocytes, muscle, mantle, gill, hepatopancreas and gonad, among which the maximum expression level were found in hemocytes. Being infected with Vibrio splendidus, Staphylococcus aureus or Yarrowia lipolytica, the mRNA expression levels of CfDAD1 in hemocytes were all significantly up-regulated. Moreover, the apoptosis level of CfDAD1-suppressed group was significant higher than those of control groups. All these results indicated that CfDAD1 was efficient negative regulator of apoptosis and involved in the innate immune responses of scallop. |
| 关键词 | Apoptosis Chlamys farreri Innate immunity |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[U1706209]###428; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ07]###2211; Science and Technology Service Network Plan (STS) Major Deployment Project[KFZD-SW-106]###430; STS Regional Centre Project (Fujian province) from Chinese Academy of Sciences###431; National Natural Science Foundation of China[U1706209]; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ07]; Science and Technology Service Network Plan (STS) Major Deployment Project[KFZD-SW-106]; STS Regional Centre Project (Fujian province) from Chinese Academy of Sciences |
| WOS研究方向 | Immunology ; Zoology |
| WOS类目 | Immunology ; Zoology |
| WOS记录号 | WOS:000443548300005 |
| 出版者 | INVERTEBRATE SURVIVAL JOURNAL |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160028 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, L. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266400, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Wang, M. Q.,Wang, B. J.,Liu, M.,et al. Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:294-301. |
| APA | Wang, M. Q.,Wang, B. J.,Liu, M.,Jiang, K. Y.,&Wang, L..(2018).Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,294-301. |
| MLA | Wang, M. Q.,et al."Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):294-301. |
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| Molecular characteri（609KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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