Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda | |
Lv, Xinjia1,2,3 ; Li, Shihao1,2; Zhang, Chengsong1,2; Xiang, Jianhai1,2; Li, Fuhua1,2
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| 2018-05-01 | |
| 发表期刊 | MARINE DRUGS
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| ISSN | 1660-3397 |
| 卷号 | 16期号:5页码:13 |
| 通讯作者 | Li, Shihao([email protected]) ; Li, Fuhua([email protected]) |
| 摘要 | As a kind of antimicrobial peptides (AMP) in crustacean, anti-lipopolysaccharide factors (ALFs) have broad spectrum antimicrobial activities. In the present study, we identified four ALF genes, EcALF2-5, from the ridgetail prawn Exopalaemon carinicauda. Tissue distribution analysis showed that EcALF2 and EcALF4 transcripts were mainly located in gill, epidermis, and stomach, while EcALF3 and EcALF5 were mainly in hemocytes. Peptides corresponding to the LPS binding domain (LBD) of EcALFs were synthesized for analyzing their antimicrobial activities. Minimal inhibitory concentration (MIC) analysis showed that the synthetic LBD peptides of EcALF3 and EcALF4 could inhibit the growth of Gram-positive and Gram-negative bacteria, while the synthetic LBD peptides of EcALF2 and EcALF5 showed antibacterial activity against Vibrio. Incubation of white spot syndrome virus (WSSV) with the synthetic LBD peptides of EcALF3, EcALF4, and EcALF5 could reduce the in vivo viral copy number in WSSV-infected prawns. After silencing of EcALFs, Vibrio exhibited a rapid proliferation in the hepatopancreas of the prawn. The present data showed the important function of different EcALFs in modulating the in vivo bacterial and viral propagation in E. carinicauda. This study will provide new clues into the disease control in aquaculture. |
| 关键词 | anti-lipopolysaccharide factor antimicrobial RNA interference flora balance crustacean |
| DOI | 10.3390/md16050145 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | General Program of National Natural Science Foundation of China[41376165]###2021; General Program of National Natural Science Foundation of China[41776158]###2022; General Program of National Natural Science Foundation of China[31772880]###2023; China Agriculture Research system-48 (CARS-48)###148; General Program of National Natural Science Foundation of China[41376165]###2021; General Program of National Natural Science Foundation of China[41776158]###2022; General Program of National Natural Science Foundation of China[31772880]###2023; China Agriculture Research system-48 (CARS-48)###148; General Program of National Natural Science Foundation of China[41376165]; General Program of National Natural Science Foundation of China[41776158]; General Program of National Natural Science Foundation of China[31772880]; China Agriculture Research system-48 (CARS-48) |
| WOS研究方向 | Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Medicinal |
| WOS记录号 | WOS:000435195100008 |
| 出版者 | MDPI |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/159504 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, Shihao; Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Lv, Xinjia,Li, Shihao,Zhang, Chengsong,et al. Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda[J]. MARINE DRUGS,2018,16(5):13. |
| APA | Lv, Xinjia,Li, Shihao,Zhang, Chengsong,Xiang, Jianhai,&Li, Fuhua.(2018).Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda.MARINE DRUGS,16(5),13. |
| MLA | Lv, Xinjia,et al."Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda".MARINE DRUGS 16.5(2018):13. |
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| Multiple Isoforms of（3155KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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