Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis | |
Cao, Ruobing1,2,3; Jin, Weihua4; Shan, Yeqi1,2,3; Wang, Ju1,2,3 ; Liu, Ge1,2,3; Kuang, Shan1,2; Sun, Chaomin1,2
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| 2018-03-01 | |
| 发表期刊 | MARINE DRUGS
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| ISSN | 1660-3397 |
| 卷号 | 16期号:3页码:18 |
| 通讯作者 | Sun, Chaomin([email protected]) |
| 摘要 | Tumor cells that acquire metastatic potential have developed resistance to anoikis, a cell death process, after detachment from their primary site to the second organ. In this study, we investigated the molecular mechanisms of a novel marine bacterial polysaccharide EPS11 which exerts its cytotoxic effects through affecting cancer cell adhesion and anoikis. Firstly, we found that EPS11 could significantly affect cell proliferation and block cell adhesion in A549 cells. We further demonstrated that the expression of several cell adhesion associated proteins is downregulated and the filiform structures of cancer cells are destroyed after EPS11 treatment. Interestingly, the destruction of filiform structures in A549 cells by EPS11 is in a dose-dependent manner, and the inhibitory tendency is very consistent with that observed in the cell adhesion assay, which confirms that filiform structures play important roles in modulating cell adhesion. Moreover, we showed that EPS11 induces apoptosis of A549 cells through stimulating beta III-tubulin associated anoikis: (i) EPS11 inhibits the expression of beta III-tubulin in both transcription and translation levels; and (ii) EPS11 treatment dramatically decreases the phosphorylation of protein kinase B (PKB or AKT), a critical downstream effector of beta III-tubulin. Importantly, EPS11 evidently inhibits the growth of A549-derived tumor xenografts in vivo. Thus, our results suggest that EPS11 may be a potential candidate for human non-small cell lung carcinoma treatment via blocking filiform structure mediated adhesion and stimulating beta III-tubulin associated anoikis. |
| 关键词 | polysaccharide cancer filiform structures beta III-tubulin anoikis adhesion |
| DOI | 10.3390/md16030085 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Natural Science Outstanding Youth Fund of Shandong Province[JQ201607]###1593; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology[2015ASTP]###727; Taishan Young Scholar Program of Shandong Province###1878; 100-Talent Project of Chinese Academy of Sciences###1879; Natural Science Outstanding Youth Fund of Shandong Province[JQ201607]; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology[2015ASTP]; Taishan Young Scholar Program of Shandong Province; 100-Talent Project of Chinese Academy of Sciences |
| WOS研究方向 | Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Medicinal |
| WOS记录号 | WOS:000428510200011 |
| 出版者 | MDPI |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/159206 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Chaomin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Dept Earth Sci, Beijing 100049, Peoples R China 4.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310014, Zhejiang, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 通讯作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Cao, Ruobing,Jin, Weihua,Shan, Yeqi,et al. Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis[J]. MARINE DRUGS,2018,16(3):18. |
| APA | Cao, Ruobing.,Jin, Weihua.,Shan, Yeqi.,Wang, Ju.,Liu, Ge.,...&Sun, Chaomin.(2018).Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis.MARINE DRUGS,16(3),18. |
| MLA | Cao, Ruobing,et al."Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis".MARINE DRUGS 16.3(2018):18. |
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