Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF | |
Gu, Han-jie1,2,3,4; Sun, Qing-lei1,2,3 ; Jiang, Shuai1,2,3; Zhang, Jian1,2,3,5; Sun, Li1,2,3
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| 2018-07-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| 卷号 | 84页码:382-395 |
| 通讯作者 | Zhang, Jian([email protected]) ; Sun, Li([email protected]) |
| 摘要 | Anti-lipopolysaccharide factor (ALF) is a type of antimicrobial peptides (AMPs) with a vital role in antimicrobial defense. Although a large amount of ALFs have been identified from neritic and fresh water crustacean species, no functional investigation of ALFs from deep-sea animals have been documented. In the present study, we characterized the immune function of an ALF molecule (named RspALF1) from the shrimp Rimicaris sp. residing in the deep-sea hydrothermal vent in Desmos, Manus Basin. RspALF1 shares 51.5%-62.4% overall sequence identities with known shrimp ALFs and contains the conserved LPS binding domain (LBD). Both recombinant RspALF1 (rRspALF1) and the LBD-derived peptide (ALF1P1) bound to the cell wall components of Gram-negative and Gram-positive bacteria and killed a wide range of bacteria, especially those from deep-sea hydrothermal field, by damaging bacterial cellular structures. The bactericidal activities of rRspALF1 and ALF1P1 were optimal and stably maintained from 4 C to 37 C, which is comparable to the ambient temperature range of the habitat of Rimicaris sp. In addition to bacteria, rRspALF1 and ALF1P1 also exhibited anti-fungal activity. rRspALF1 and ALF1P1 exhibited high killing efficiencies, which, in terms of MIC values, were ranged between 0.25 M and 4 M for bacteria and 4 mu M-81.1 mu M for fungi. When introduced in vivo, both rRspALF1 and ALF1P1 effectively inhibited bacterial infection in shrimp and reduced the dissemination of bacterial and viral pathogens in fish. Together, these results provide the first insight into the biological property of deep-sea ALF and indicate that RspALF1 very likely plays a significant role in immune defense by functioning as a highly effective antimicrobial with a broad target range. (C) 2018 Elsevier Ltd. All rights reserved. |
| 关键词 | Anti-lipopolysaccharide factor Antimicrobial peptides Rimicaris sp. Deep-sea hydrothermal vent Antibacterial Antiviral |
| DOI | 10.1016/j.dci.2018.03.016 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Strategic Priority Research Program of Chinese Academy of Sciences[XDA11030401]###1761; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2016ASKJ14]###615; AoShan Talents Program by Qingdao National Laboratory for Marine Science and Technology[2015ASTP]###1762; Taishan Scholar Program of Shandong Province###423; Strategic Priority Research Program of Chinese Academy of Sciences[XDA11030401]; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2016ASKJ14]; AoShan Talents Program by Qingdao National Laboratory for Marine Science and Technology[2015ASTP]; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| WOS类目 | Fisheries ; Immunology ; Zoology |
| WOS记录号 | WOS:000431160800037 |
| 出版者 | ELSEVIER SCI LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158927 |
| 专题 | 实验海洋生物学重点实验室 深海极端环境与生命过程研究中心 |
| 通讯作者 | Zhang, Jian; Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Han-jie,Sun, Qing-lei,Jiang, Shuai,et al. First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,84:382-395. |
| APA | Gu, Han-jie,Sun, Qing-lei,Jiang, Shuai,Zhang, Jian,&Sun, Li.(2018).First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,84,382-395. |
| MLA | Gu, Han-jie,et al."First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 84(2018):382-395. |
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