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| Identification and characterization two isoforms of NADH:ubiquinone oxidoreductase from the hyperthermophilic eubacterium Aquifex aeolicus | |
| Peng, Guohong1,5; Meyer, Bjorn2; Sokolova, Lucie3; Liu, Wenxia1; Bornemann, Sandra2; Juli, Jana1; Zwicker, Klaus4; Karas, Michael2; Brutschy, Bernd3; Michel, Hartmut1 | |
| 2018-05-01 | |
| 发表期刊 | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
 |
| ISSN | 0005-2728 |
| 卷号 | 1859期号:5页码:366-373 |
| 通讯作者 | Peng, Guohong([email protected]) ; Michel, Hartmut([email protected]) |
| 摘要 | The NADH:ubiquinone oxidoreductase (complex I) is the first enzyme of the respiratory chain and the entry point for most electrons. Generally, the bacterial complex I consists of 14 core subunits, homologues of which are also found in complex I of mitochondria. In complex I preparations from the hyperthermophilic bacterium Aquifex aeolicus we have identified 20 partially homologous subunits by combining MALDI-TOF and LILBID mass spectrometry methods. The subunits could be assigned to two different complex I isoforms, named NQOR1 and NQOR2. NQOR1 consists of subunits NuoA(2), NuoB, NuoD(2), NuoE, NuoF, NuoG, Nuol(1), NuoH(1), NuoJ(1), NuoL(1), NuoM(1) and NuoN(1), with an entire mass of 504.17 kDa. NQOR2 comprises subunits NuoA(1), NuoB, NuoD(1), NuoE, NuoF, NuoG, NuoH(2), NuoI(2), NuoJ(1), NuoK(1), NuoL(2), NuoM(2) and NuoN(2), with a total mass of 523.99 kDa. Three Fe-S clusters could be identified by EPR spectroscopy in a preparation containing predominantly NQOR1. These were tentatively assigned to a binuclear center N1, and two tetranuclear centers, N2 and N4. The redox midpoint potentials of N1 and N2 are 273 mV and 184 mV, respectively. Specific activity assays indicated that NQOR1 from cells grown under low concentrations of oxygen was the more active form. Increasing the concentration of oxygen in the bacterial cultures induced formation of NQOR2 showing the lower specific activity. |
| 关键词 | Aquifex aeolicus Complex I Isoform enzyme NADH:ubiquinone oxidoreductase Bacteria |
| DOI | 10.1016/j.bbabio.2018.02.008 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Deutsche Forschungsgemeinschaft[SFB 628]###834; Deutsche Forschungsgemeinschaft (Cluster of Excellence Macromolecular Complexes Frankfurt)###835; Max-Planck-Gesellschaft###836; Deutsche Forschungsgemeinschaft[SFB 628]; Deutsche Forschungsgemeinschaft (Cluster of Excellence Macromolecular Complexes Frankfurt); Max-Planck-Gesellschaft |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| WOS记录号 | WOS:000430881200007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158924 |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Peng, Guohong; Michel, Hartmut |
| 作者单位 | 1.Max Planck Inst Biophys, Dept Mol Membrane Biol, D-60438 Frankfurt, Germany 2.Goethe Univ, Inst Pharmaceut Chem, D-60438 Frankfurt, Germany 3.Goethe Univ, Inst Phys & Theoret Chem, D-60438 Frankfurt, Germany 4.Goethe Univ Frankfurt, Inst Biochem 1, Fac Med, D-60590 Frankfurt, Germany 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Peng, Guohong,Meyer, Bjorn,Sokolova, Lucie,et al. Identification and characterization two isoforms of NADH:ubiquinone oxidoreductase from the hyperthermophilic eubacterium Aquifex aeolicus[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2018,1859(5):366-373. |
| APA | Peng, Guohong.,Meyer, Bjorn.,Sokolova, Lucie.,Liu, Wenxia.,Bornemann, Sandra.,...&Michel, Hartmut.(2018).Identification and characterization two isoforms of NADH:ubiquinone oxidoreductase from the hyperthermophilic eubacterium Aquifex aeolicus.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1859(5),366-373. |
| MLA | Peng, Guohong,et al."Identification and characterization two isoforms of NADH:ubiquinone oxidoreductase from the hyperthermophilic eubacterium Aquifex aeolicus".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1859.5(2018):366-373. |
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| Identification and c（932KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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