Knowledge Management System Of Institute of Oceanology, Chinese Academy of Sciences
| Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China | |
Fu, Songzhe1; Wang, Liping2; Tian, Huiqin3; Wei, Dawei4; Liu, Ying1
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| 2018-04-01 | |
| 发表期刊 | ANNALS OF MICROBIOLOGY
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| ISSN | 1590-4261 |
| 卷号 | 68期号:4页码:175-184 |
| 通讯作者 | Fu, Songzhe([email protected]) |
| 摘要 | Acute hepatopancreatic necrosis disease (AHPND) outbreaks in cultured shrimps were identified in Zhangpu, China. One Vibrio parahaemolyticus strain PB1937 was isolated from the cultured shrimps and was confirmed as a causative agent of the AHPND outbreak by employing Koch's four postulates. Challenge tests with 10(6) cells ml(-1) of strain PB1937 caused 100% mortality of shrimps, indicating it had sufficient virulence to cause the outbreak. Phylogenomic analysis revealed a clear divergence between PB1937 and 14 publicly available V. parahaemolyticus strains and divided 11 AHPND-causing strains into six genomic clusters. Prophage profiling of above strains revealed strong correlations with their genomic relationship, while Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) were almost absent in the genomes. The binary toxin gene pirAB(vp) directly related to the development of AHPND was found in a 70-kb plasmid p1937-1 in PB1937 but was absent in a 78-kb novel plasmid p1937-2, which shared 46% sequence similarity with p1937-1. Comparative genomic analysis revealed that PB1937 has a novel truncated type VI secretion system (T6SS1) which possibly affects its antibacterial activity. In addition, three novel genomic islands were reported. The analysis of the genomes gave some clues regarding the correlation of virulence with its genomic trait for the AHPND strains. |
| 关键词 | Vibrio parahaemolyticus AHPND PacBio pirAB(vp) Prophage Type VI secretion system |
| DOI | 10.1007/s13213-018-1328-0 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key R&D Program of China[2017YFD0701700]###440; National Natural Science Foundation of China[31672673]###1742; National Natural Science Foundation of China[31472312]###66; National Key R&D Program of China[2017YFD0701700]; National Natural Science Foundation of China[31672673]; National Natural Science Foundation of China[31472312] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000429125500002 |
| 出版者 | SPRINGER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158774 |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Fu, Songzhe |
| 作者单位 | 1.Dalian Ocean Univ, Coll Marine Technol & Environm, 52 Heishijiao St, Dalian 116023, Peoples R China 2.State Oceanog Adm China, Inst Oceanog 3, Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Northwest Agr & Forestry Univ, Coll Life Sci, Yangling 712100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Songzhe,Wang, Liping,Tian, Huiqin,et al. Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China[J]. ANNALS OF MICROBIOLOGY,2018,68(4):175-184. |
| APA | Fu, Songzhe,Wang, Liping,Tian, Huiqin,Wei, Dawei,&Liu, Ying.(2018).Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China.ANNALS OF MICROBIOLOGY,68(4),175-184. |
| MLA | Fu, Songzhe,et al."Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China".ANNALS OF MICROBIOLOGY 68.4(2018):175-184. |
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| Pathogenicity and ge（715KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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