Description of a Zostera marina catalase gene involved in responses to temperature stress

doi:10.7717/peerj.4532

IOCAS-IR

	Description of a Zostera marina catalase gene involved in responses to temperature stress
	Zang, Yu 1; Liu, Jiao 3; Tang, Xue Xi 1,2; Zhou, Bin 1,2
	2018-03-26
发表期刊	PEERJ
ISSN	2167-8359
卷号	6 页码:20
通讯作者	Zhou, Bin([email protected])
摘要	Catalase (CAT) is an antioxidant enzyme that plays a significant role in cellular protection against oxidative darnage by degradation of hydrogen peroxide to oxygen and water. In the present study, the complete CAT cDNA sequence of Zostera marina was identified through expressed sequence tags (EST) analysis and the rapid amplification of cDNA ends (RACE) technique. The nucleotide sequence of ZmCAT cDNA consisted of 1,816 by with a 1,434 bp open reading frame (ORF) encoding a polypeptide of 477 amino acid residues, which possessed significant homology to other known plant CATs. The molecular mass of the predicted protein was 55.3 kDa with an estimated isoelectric point of 6.40. Phylogenetic analysis showed that ZmCAT was closely related to CAT from gramineous species. In response to temperature stress, H2O2 and MDA contents in Z. marina-increased significantly with cold stress (<10 degrees C) and heat stress (>25 degrees C). ZmCAT expression was significantly upregulated at temperatures from 5 to 10 degrees C and then gradually downregulated, reaching its lowest expression at 30 degrees C. Recombinant ZmCAT protein exhibited strong antioxidant activity over a wide temperature range, with the highest rZmCAT activity observed at 25 degrees C and a higher relative activity retained even with heat stress. All these results indicated that ZmCAT was a member of the plant CAT family and involved in minimizing oxidative damage effects in Z. marina under temperature stress.
关键词	CAT Temperature stress mRNA expression Enzyme activity
DOI	10.7717/peerj.4532
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41476091]###1738; Key Special Program on Marine Environmental Security Protection'' of National Research and Development Project of China[2016YFC1402305]###1739; National Natural Science Foundation of China[41476091]; Key Special Program on Marine Environmental Security Protection'' of National Research and Development Project of China[2016YFC1402305]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000428696100002
出版者	PEERJ INC
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158765
专题	中国科学院海洋研究所
通讯作者	Zhou, Bin
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zang, Yu,Liu, Jiao,Tang, Xue Xi,et al. Description of a Zostera marina catalase gene involved in responses to temperature stress[J]. PEERJ,2018,6:20.
APA	Zang, Yu,Liu, Jiao,Tang, Xue Xi,&Zhou, Bin.(2018).Description of a Zostera marina catalase gene involved in responses to temperature stress.PEERJ,6,20.
MLA	Zang, Yu,et al."Description of a Zostera marina catalase gene involved in responses to temperature stress".PEERJ 6(2018):20.

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