Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei)

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	Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei)
	Liu, X.1,2,3 ; Wang, M.1,2; Shao, J.1,2,3; Wang, B.1,2; Jiang, K.1,2; Liu, M.1,2; Wang, L.1,2
	2018
发表期刊	ISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN	1824-307X
卷号	15 页码:52-60
通讯作者	Liu, M.([email protected]) ; Wang, L.([email protected])
摘要	The Ras family GTPase Rheb, a GTP-binding protein, binds specifically to the mTOR catalytic domain and induces activation of the mTOR catalytic function. Furthermore, Rheb is related to a stable modification of the configuration of mTORC1 that increases access of substrates to their binding site on the raptor polypeptide. In the present research, a cDNA of 898 bp for the Litopenaeus vannamei Rheb was cloned via rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of Rheb contained an open reading frame (ORF) of 549 bp, which encoded a protein of 182 amino acids. The amino acid sequence of Rheb shared more than 60% similarity with other identified Rheb proteins. A Ras domain (from P3 to A169) was found in the amino acid sequence of Rheb that can react selectively and non-covalently with GTP. The mRNA transcripts of Rheb were consistently expressed in all the tested tissues, including muscle, gill, hepatopancreas, eyestalk, intestine and stomach. The mRNA expression profiles of Rheb in muscle after the stimulation with rapamycin were promoted, which further proved that Rheb protein could be a feedback regulator to mTOR signaling pathway. Furthermore, the results of the present study indicated that Rheb played an important role in the regulation of mTOR signaling pathway during the stimulation of dietary restriction, amino acid supplementation and rapamycin stimulation in shrimp.
关键词	Litopenaeus vannamei molecular cloning mTOR Rheb
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41406151]###1614; Chinese Academy of Sciences STS Major Deployment Project[KFZD-SW-106]###1615; National Natural Science Foundation of China[41406151]; Chinese Academy of Sciences STS Major Deployment Project[KFZD-SW-106]
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000428589300002
出版者	INVERTEBRATE SURVIVAL JOURNAL
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158460
专题	实验海洋生物学重点实验室
通讯作者	Liu, M.; Wang, L.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Liu, X.,Wang, M.,Shao, J.,et al. Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei)[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:52-60.
APA	Liu, X..,Wang, M..,Shao, J..,Wang, B..,Jiang, K..,...&Wang, L..(2018).Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei).ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,52-60.
MLA	Liu, X.,et al."Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei)".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):52-60.

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