Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli

doi:10.1186/s12934-018-0900-4

IOCAS-IR

	Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli
	Liu, Min 1,2; Ding, Yamei 3; Xian, Mo 1; Zhao, Guang 1,2
	2018-03-28
发表期刊	MICROBIAL CELL FACTORIES
ISSN	1475-2859
卷号	17 页码:11
通讯作者	Xian, Mo([email protected]) ; Zhao, Guang([email protected])
摘要	Background: Glycolate is a valuable chemical with extensive applications in many different fields. The traditional methods to synthesize glycolate are quite expensive and toxic. So, the biotechnological production of glycolate from sustainable feedstocks is of interest for its potential economic and environmental advantages. D-Xylose is the second most abundant sugar in nature and accounts for 18-30% of sugar in lignocellulose. New routes for the conversion of xylose to glycolate were explored. Results: Overexpression of aceA and ghrA and deletion of aceB in Escherichia coli were examined for glycolate production from xylose, but the conversion was initially ineffective. Then, a new route for glycolate production was established in E. coli by introducing-NAD(+)-dependent xylose dehydrogenase (xdh) and xylonolactonase (xylC) from Caulobacter crescentus. The constructed engineered strain Q2562 produced 28.82 +/- 0.56 g/L glycolate from xylose with 0.60 +/- 0.01 g/L/h productivity and 0.38 +/- 0.07 g/g xylose yield. However, 27.18 +/- 2.13 g/L acetate was accumulated after fermentation. Deletions of iclR and ackA were used to overcome the acetate excretion. An ackA knockout resulted in about 66% decrease in acetate formation. The final engineered strain Q2742 produced 43.60 +/- 1.22 g/L glycolate, with 0.91 +/- 0.02 g/L/h productivity and 0.46 +/- 0.03 g/g xylose yield. Conclusions: A new route for glycolate production from xylose was established, and an engineered strain Q2742 was constructed from this new explored pathway. The engineering strain showed the highest reported productivity of glycolate to date. This research opened up a new prospect for bio-refinery of xylose and an alternative choice for industrial production of glycolate.
关键词	Xylose Glycolate Glycolaldehyde Glyoxylate Escherichia coli
DOI	10.1186/s12934-018-0900-4
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31670089]###1601; National Natural Science Foundation of China[31722001]###1602; China Postdoctoral Science Foundation[2016M600564]###1603; Postdoctoral Applied Research Project of Qingdao###1604; National Natural Science Foundation of China[31670089]; National Natural Science Foundation of China[31722001]; China Postdoctoral Science Foundation[2016M600564]; Postdoctoral Applied Research Project of Qingdao
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000428910100002
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158451
专题	中国科学院海洋研究所
通讯作者	Xian, Mo; Zhao, Guang
作者单位	1.Chinese Acad Siences, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Min,Ding, Yamei,Xian, Mo,et al. Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli[J]. MICROBIAL CELL FACTORIES,2018,17:11.
APA	Liu, Min,Ding, Yamei,Xian, Mo,&Zhao, Guang.(2018).Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli.MICROBIAL CELL FACTORIES,17,11.
MLA	Liu, Min,et al."Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli".MICROBIAL CELL FACTORIES 17(2018):11.

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