A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas

doi:10.1016/j.dci.2017.12.029

IOCAS-IR > 实验海洋生物学重点实验室

	A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas
	Jia, Yunke 1,3; Yang, Bin 1,3; Dong, Wenjing1,3 ; Liu, Zhaoqun4 ; Lv, Zhao 1,3; Jia, Zhihao 1,3; Qiu, Limei 1; Wang, Lingling 2,4; Song, Linsheng 2,4
	2018-05-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	82 页码:83-93
通讯作者	Wang, Lingling([email protected]) ; Song, Linsheng()
摘要	Serotonin receptors, including ligand-gated ion channel (LGICs) and G protein-coupled receptors (GPCR), play vital roles in modulating physiological processes and immunoreaction. In the present study, a homologue of serotonin (5-HT) receptor was identified from oyster Crassostrea gigas (designated Cg5-HTR1). Its open reading frame (ORF) was of 1239 bp, encoding-a polypeptide of 412 amino acids with a seven transmembrane region. Cg5-HTR-1 shared high similarity with the 5-HTRs from other animals. The cAMP contents in HEK293T cells decreased significantly after Cg5-HTR-1 transfection and 5-HT incubation (p < .05), while blocking Cg5-HTR-1 with specific receptor antagonist reversed this downtrend. The intracellular Ca2+ concentrations increased significantly (p < .05) after cell transfection and 5-HT incubation, and the antagonist treatment also arrested this process. Cg5-HTR-1 transcripts were widely distributed in various tissues, with the highest level in hepatopancreas and lowest level in mantle and gill. The mRNA expression of Cg5-HTR-1 in hemocyte increased significantly after lipopolysaccharide (LPS) stimulation and reached the peak level (6.47-fold, p < .05) at 6 h post treatment. The inhibition of Cg5-HTR-1 significantly reduced the expression of tumor necrosis factor (TNF) mRNA in hemocyte, down-regulated the superoxide dismutase (SOD) activity in serum, and induced the apoptosis of hemocyte (p < .05). These results suggested that Cg5-HTR-1 was a novel member of 5-HT1 receptor family and it mediated serotonergic immunomodulation on both cellular and humoral immune responses. (C) 2018 Elsevier Ltd. All rights reserved.
关键词	Serotonin Serotonin receptor 1A Cellular immune response Humoral immune response Crassostrea gigas
DOI	10.1016/j.dci.2017.12.029
收录类别	SCI
语种	英语
资助项目	National Science Foundation of China[U1706204]###733; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13]###1399; Dalian High Level Talent Innovation Support Program[2015R020]###742; Earmarked Fund from Modern Agro-industry Technology Research System[CARS-49]###1504; Research Foundation for Distinguished Professor in Liaoning###737; Talented Scholars in Dalian Ocean University###741; Aoshan Talent Cultivation Program###1505; National Science Foundation of China[U1706204]; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13]; Dalian High Level Talent Innovation Support Program[2015R020]; Earmarked Fund from Modern Agro-industry Technology Research System[CARS-49]; Research Foundation for Distinguished Professor in Liaoning; Talented Scholars in Dalian Ocean University; Aoshan Talent Cultivation Program
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000427668700009
出版者	ELSEVIER SCI LTD
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158367
专题	实验海洋生物学重点实验室
通讯作者	Wang, Lingling; Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Jia, Yunke,Yang, Bin,Dong, Wenjing,et al. A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,82:83-93.
APA	Jia, Yunke.,Yang, Bin.,Dong, Wenjing.,Liu, Zhaoqun.,Lv, Zhao.,...&Song, Linsheng.(2018).A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,82,83-93.
MLA	Jia, Yunke,et al."A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 82(2018):83-93.

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