Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus | |
Fu, Yuanshuai1; Gao, Lina1; Shi, Zhiyi1 ; You, Feng2; Zhang, Junling1; Li, Wenjuan1
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| 2018-04-01 | |
| 发表期刊 | FISH PHYSIOLOGY AND BIOCHEMISTRY
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| ISSN | 0920-1742 |
| 卷号 | 44期号:2页码:451-463 |
| 通讯作者 | Shi, Zhiyi([email protected]) |
| 摘要 | Heterochronic lin-28 is a conserved RNA-binding protein that plays a key role in the timing of developmental events in organisms. As a crucial heterochronic gene, the protein controls developmental events of the second of four larval stages in Caenorhabditi elegans. Heterochronic let-7 miRNAs are often present in various species and highly conserved in sequence and biological function and are required for various biological processes. Previous studies showed that ten let-7 miRNAs were identified in the Japanese flounder (Paralichthys olivaceus) and that they were primarily expressed during metamorphosis. In this study, we clone and characterize the lin-28a gene from P. olivaceus and exhibit its dynamic expression pattern at different developmental stages and various adult tissues. The results show that the P. olivaceus lin-28a gene has high sequence similarity with other species and is highly expressed in the embryonic stage but weakly expressed in the larval stage. In addition, lin-28a overexpression causes cell proliferation and significantly promotes the levels of pre-let-7a and pre-let-7d while markedly depressing let-7a and let-7d expression in FEC (Flounder Embryonic Cell), which indicate that lin-28 possibly blocks the maturation of let-7 miRNAs. Additionally, lin-28a is identified as a target gene of let-7 miRNAs, and let-7 miRNAs directly regulate lin-28a expression by targeting its 3' UTR. Taken together, lin-28a along with let-7 miRNA participates in a lin-28/let-7 axis pathway that regulates cell division and timing of embryonic and metamorphic events in P. olivaceus. |
| 关键词 | P. olivaceus lin-28a let-7 miRNAs Embryonic development Metamorphosis |
| DOI | 10.1007/s10695-017-0445-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2015No01]###1495; National Natural Science Foundation of China[41506159]###1496; National Natural Science Foundation of China[41306128]###1497; Natural Science Foundation of Shanghai[15ZR1420600]###1498; Innovation Programme of Shanghai Municipal Education Commission[15ZZ083]###1499; Science and Technology Development Foundation of Shanghai Ocean University[A2-0203-00-100204]###1500; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2015No01]; National Natural Science Foundation of China[41506159]; National Natural Science Foundation of China[41306128]; Natural Science Foundation of Shanghai[15ZR1420600]; Innovation Programme of Shanghai Municipal Education Commission[15ZZ083]; Science and Technology Development Foundation of Shanghai Ocean University[A2-0203-00-100204] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| WOS类目 | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| WOS记录号 | WOS:000428055300003 |
| 出版者 | SPRINGER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158358 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Shi, Zhiyi |
| 作者单位 | 1.Shanghai Ocean Univ, Minist Agr, Key Lab Genet Resources Freshwater Aquaculture &, 999 Hu Cheng Huan Rd, Shanghai 201306, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Yuanshuai,Gao, Lina,Shi, Zhiyi,et al. Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2018,44(2):451-463. |
| APA | Fu, Yuanshuai,Gao, Lina,Shi, Zhiyi,You, Feng,Zhang, Junling,&Li, Wenjuan.(2018).Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus.FISH PHYSIOLOGY AND BIOCHEMISTRY,44(2),451-463. |
| MLA | Fu, Yuanshuai,et al."Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus".FISH PHYSIOLOGY AND BIOCHEMISTRY 44.2(2018):451-463. |
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| Characterization and（1851KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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