Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis | |
Li, Shihao1,2; Li, Fuhua1,2; Yu, Kuijie1; Xiang, Jianhai1
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| 2018-04-05 | |
| 发表期刊 | GENE
 |
| ISSN | 0378-1119 |
| 卷号 | 649页码:1-7 |
| 通讯作者 | Li, Fuhua([email protected]) |
| 摘要 | The doublesex and its homologue genes are important regulators of sexual differentiation which are conserved among animal kingdom. In the present study, we reported a doublesex gene (designated as FcDsx) identified from the Chinese shrimp F. chinensis. The gene structure, nucleotide and deduced amino acid sequences of FcDsx were characterized. The results showed that the deduced amino acid sequence of FcDsx had the common features of Dsx proteins, including a doublesex/male abnormal 3 (DM) domain, an oligomerization domain and a predicted monopartite nuclear localization signal. The expression patterns of FcDsx in different tissues and developmental stages were detected. FcDsx exhibited a sex-biased expression patterns in different tissues and its expression level increased along with developmental stages. In addition, its regulation on the expression of FcIAG, a gene important for sexual differentiation of male crustacean, was also analyzed. Putative Dsx binding site was identified on the promoter region of FcIAG and knockdown of FcDsx could reduce the expression of FcIAG, which suggested that FcDsx might be the upstream regulator of FcIAG. The present data indicated that FcDsx gene might involve in shrimp sexual differentiation process. |
| 关键词 | Doublesex Insulin-like androgenic gland hormone Expression Sex determination Shrimp |
| DOI | 10.1016/j.gene.2018.01.043 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31302171]###1467; National Natural Science Foundation of China[31461143007]###1468; China Agriculture Research System-48[CARS-48]###1408; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]###792; Scientific and Technological Innovation Project###54; National Natural Science Foundation of China[31302171]; National Natural Science Foundation of China[31461143007]; China Agriculture Research system-48[CARS-48]; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]; Scientific and Technological Innovation Project |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| WOS记录号 | WOS:000427314600001 |
| 出版者 | ELSEVIER SCIENCE BV |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158146 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 通讯作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Shihao,Li, Fuhua,Yu, Kuijie,et al. Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis[J]. GENE,2018,649:1-7. |
| APA | Li, Shihao,Li, Fuhua,Yu, Kuijie,&Xiang, Jianhai.(2018).Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis.GENE,649,1-7. |
| MLA | Li, Shihao,et al."Identification and characterization of a doublesex gene which regulates the expression of insulin-like androgenic gland hormone in Fenneropenaeus chinensis".GENE 649(2018):1-7. |
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| Identification and c（1409KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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