Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas | |
Sun, Rui1; Qiu, Limei1; Yi, Qilin2,3,4; Wang, Mengqiang1 ; Yue, Feng1; Wang, Lingling2,3; Song, Linsheng2,3,4
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| 2018-03-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 74页码:386-392 |
| 通讯作者 | Wang, Lingling([email protected]) |
| 摘要 | Toll like receptor (TLR) signaling cascades are under precise regulations to ensure the proper immune responses during various pathogen invasions. The neuregulin receptor degradation protein-1 (Nrdp1) has been demonstrated to be a novel negative regulator of TLR signaling by targeting MyD88 to induce degradation in mammals. In the present study, an Nrdp1 homologue, CgNrdp1, was identified from the genome of Pacific oyster Crassostrea gigas. It contained an open reading frame encoding a polypeptide of 315 amino acids which shared high identities with other homologues from different species. There was a conserved RING domain in CgNrdp1, indicating the functional E3 ubiquitin ligase activity. The bacterially expressed recombinant CgNrdp1 and CgMyD88 showed much stronger affinity compared to control groups in the ELISA assay, showing the interacting ability between CgNrdp1 and CgMyD88. When CgMyD88 or HsMyD88 was co-transfected with CgNrdp1 into HEK293T cells, the luciferase activities of NF-kappa B were significantly decreased compared to those in MyD88 single-transfection groups, indicating the conserved negative regulating function of CgNrdp1 on the MyD88 induced TLR signaling. These results indicated that CgNrdp1 was a negative regulator of TLR signaling in oyster and the Nrdp1-MyD88 axis was functional and highly conserved from mollusks to mammals in the negative regulation of TLR signaling. |
| 关键词 | Nrdp1 Toll like receptor signaling pathway Immune regulation Evolution of innate immunity Crassostrea gigas |
| DOI | 10.1016/j.fsi.2017.12.057 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Science Foundation of China[U1706204]###733; Dalian High Level Talent Innovation Support Program[2015R020]###742; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13]###1399; Research Foundation for Distinguished Professor in Liaoning###737; Talented Scholars in Dalian Ocean University###741; National Science Foundation of China[U1706204]; Dalian High Level Talent Innovation Support Program[2015R020]; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13]; Research Foundation for Distinguished Professor in Liaoning; Talented Scholars in Dalian Ocean University |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000427344200041 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158123 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Lingling |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun, Rui,Qiu, Limei,Yi, Qilin,et al. CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:386-392. |
| APA | Sun, Rui.,Qiu, Limei.,Yi, Qilin.,Wang, Mengqiang.,Yue, Feng.,...&Song, Linsheng.(2018).CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,74,386-392. |
| MLA | Sun, Rui,et al."CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 74(2018):386-392. |
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| CgNrdp1, a conserved（602KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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