CPAP3 proteins in the mineralized cuticle of a decapod crustacean

doi:10.1038/s41598-018-20835-x

IOCAS-IR > 实验海洋生物学重点实验室

	CPAP3 proteins in the mineralized cuticle of a decapod crustacean
	Abehsera, Shai 1,2; Zaccai, Shir 1; Mittelman, Binyamin 1,2; Glazer, Lilah 4; Weil, Simy 1,2; Khalaila, Isam 2,3; Davidov, Geula 1,2; Bitton, Ronit 1,2; Zarivach, Raz 1,2; Li, Shihao 5; Li, Fuhua 5; Xiang, Jianhai5 ; Manor, Rivka 1,2; Aflalo, Eliahu D.1,2; Sagi, Amir 1,2
	2018-02-05
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	8 页码:12
通讯作者	Sagi, Amir([email protected])
摘要	The pancrustacean theory groups crustaceans and hexapods (once thought to comprise separate clades within the Arthropoda) into a single clade. A key feature common to all pancrustaceans is their chitinous exoskeleton, with a major contribution by cuticular proteins. Among these, are the CPAP3' s, a family of cuticular proteins, first identified in the hexapod Drosophila melanogaster and characterized by an N-terminal signaling peptide and three chitin-binding domains. In this study, CPAP3 proteins were mined from a transcriptomic library of a decapod crustacean, the crayfish Cherax quadricarinatus. Phylogenetic analysis of other CPAP3 proteins from hexapods and other crustaceans showed a high degree of conservation. Characterization of the crayfish proteins, designated CqCPAP3's, suggested a major role for CPAP3'sin cuticle formation. Loss-of-function experiments using RNAi supported such a notion by demonstrating crucial roles for several CqCPAP3 proteins during molting. A putative mode of action for the CqCPAP3 proteins-theoretically binding three chitin strands-was suggested by the structural data obtained from a representative recombinant CqCPAP3. The similarities between the CqCPAP3 proteins and their hexapod homologues further demonstrated common genetic and proteinaceous features of cuticle formation in pancrustaceans, thereby reinforcing the linkage between these two highly important phylogenetic groups.
DOI	10.1038/s41598-018-20835-x
收录类别	SCI
语种	英语
资助项目	Israel Science Foundation[613/13]###1411; Israel Science Foundation###1412; National Natural Science Foundation of China[605/14]###1413; National Institute for Biotechnology in the Negev (NIBN)###1414; Israel Science Foundation[613/13]; Israel Science Foundation; National Natural Science Foundation of China[605/14]; National Institute for Biotechnology in the Negev (NIBN)
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000424087700102
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158070
专题	实验海洋生物学重点实验室
通讯作者	Sagi, Amir
作者单位	1.Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel 2.Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, Beer Sheva, Israel 3.Ben Gurion Univ Negev, Dept Biotechnol Engn, Beer Sheva, Israel 4.Duke Univ, Med Ctr, Dept Psychiat & Behav Sci, Durham, NC USA 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Abehsera, Shai,Zaccai, Shir,Mittelman, Binyamin,et al. CPAP3 proteins in the mineralized cuticle of a decapod crustacean[J]. SCIENTIFIC REPORTS,2018,8:12.
APA	Abehsera, Shai.,Zaccai, Shir.,Mittelman, Binyamin.,Glazer, Lilah.,Weil, Simy.,...&Sagi, Amir.(2018).CPAP3 proteins in the mineralized cuticle of a decapod crustacean.SCIENTIFIC REPORTS,8,12.
MLA	Abehsera, Shai,et al."CPAP3 proteins in the mineralized cuticle of a decapod crustacean".SCIENTIFIC REPORTS 8(2018):12.

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