Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit

doi:10.1080/21655979.2017.1321282

IOCAS-IR > 实验海洋生物学重点实验室

	Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit
	Wu, Jing1,2,3 ; Chen, Huaxin 1,2; Jiang, Peng 1,2
	2018
发表期刊	BIOENGINEERED
ISSN	2165-5979
卷号	9 期号:1 页码:108-115
通讯作者	Chen, Huaxin([email protected]) ; Jiang, Peng([email protected])
摘要	The fusion protein (SLA) of streptavidin and allophycocyanin subunit (holo-ApcA) was biosynthesized in Escherichia coli by a dual plasmid system. The recombinant SLA, purified by affinity chromatography, showed spectral properties similar to natural allophycocyanin subunit (ApcA). Spectral and Zinc staining analysis indicated that the recombinant SLA covalently bound phycocyanobilin (PCB). To improve chromophorylation rate of recombinant SLA, an in vitro chromophore attachment reaction system was established, which contained partially chromophylated SLA, PCB and lyase CpcS. Spectral analysis showed that PCB bound to the recombinant SLA rapidly during the reaction. The chromophorylation rate of SLA was improved from 21.1% to 86.5%. Immunofluorescence assay showed that SLA with high chromophorylation rate had higher detection signal. Thus, in vitro chromophore attachment is an effective way to improve the chromophorylation rate of recombinant phycobiliprotein.
关键词	Allophycocyanin Chromophore attachment Chromophorylation rate Immunofluorescence assay Phycobiliprotein
DOI	10.1080/21655979.2017.1321282
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation[41276164]###1395; National 863 Program[2014AA093505]###575; National 863 Program[2014AA093501]###576; Development Funds of Science and Technology of Shinan District[2016-3-010-ZH]###574; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]###218; National Natural Science Foundation[41276164]; National 863 Program[2014AA093505]; National 863 Program[2014AA093501]; development funds of science and technology of Shinan district[2016-3-010-ZH]; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000425053900001
出版者	TAYLOR & FRANCIS INC
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158039
专题	实验海洋生物学重点实验室
通讯作者	Chen, Huaxin; Jiang, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wu, Jing,Chen, Huaxin,Jiang, Peng. Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit[J]. BIOENGINEERED,2018,9(1):108-115.
APA	Wu, Jing,Chen, Huaxin,&Jiang, Peng.(2018).Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit.BIOENGINEERED,9(1),108-115.
MLA	Wu, Jing,et al."Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit".BIOENGINEERED 9.1(2018):108-115.

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