Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri | |
Wang, Mengqiang1 ; Wang, Baojie1; Jiang, Keyong1; Liu, Mei1; Shi, Xiaowei1,3,4; Wang, Lei1,2
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| 2018 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 72页码:282-290 |
| 通讯作者 | Wang, Lei([email protected]) |
| 摘要 | Superoxide dismutase (SOD) ubiquitously found in both prokaryotes and eukaryotes functions as the first and essential enzyme in the antioxidant system. In the present study, a manganese SOD (designated as CfmtMnSOD) was cloned from Zhikong scallop Chlamys farreri. The complete cDNA sequence of CfmtMnSOD contained a 681 bp open reading frame (ORF), encoding a peptide of 226 amino acids. A SOD_Fe_N domain and a SOD_Fe_C domain were found in the deduced amino acid sequence of CfmtMnSOD. The mRNA transcripts of CfmtMnSOD were constitutively expressed in all the tested tissues, including gill, gonad, hepatopancreas, hemocytes, mantle and muscle, with the highest expression level in hemocytes. After the stimulation of Vibrio splendidus, Staphylococcus aureus and Yarrowia lipolytica, the mRNA transcripts of CfmtMnSOD in hemocytes all significantly increased. The purified rCfmtMnSOD protein exhibited Mn2+ dependent specific and low stable enzymatic activities. After Vibrio challenge, the cumulative mortality of CfmtMnSOD-suppressed scallops was significantly higher than those of control groups and the semi-lethal time for CfmtMnSOD-suppressed scallops was rather shorter than those of control groups either. Moreover, the final mortality rate of CfmtMnSOD-suppressed group was significant higher than those of control groups, even without Vibrio challenge. All these results indicated that CfmtMnSOD was efficient antioxidant enzyme involved in the innate immunity, and also essential for the survival of C. farreri. |
| 关键词 | Chlamys farreri Innate immunity Manganese superoxide dismutase |
| DOI | 10.1016/j.fsi.2017.11.010 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106]###819; Major Projects of Shandong Province[2015ZDZX05002]###1188; China Postdoctoral Science Foundation[2017M610427]###1100; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106]; Major Projects of Shandong Province[2015ZDZX05002]; China Postdoctoral Science Foundation[2017M610427] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000423644600033 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158005 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Linyi Univ, Linyi 276000, Peoples R China 4.Shandong Prov Engn Technol Res Ctr Liman Chinese, Linyi 276000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Mengqiang,Wang, Baojie,Jiang, Keyong,et al. A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:282-290. |
| APA | Wang, Mengqiang,Wang, Baojie,Jiang, Keyong,Liu, Mei,Shi, Xiaowei,&Wang, Lei.(2018).A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,72,282-290. |
| MLA | Wang, Mengqiang,et al."A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 72(2018):282-290. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A mitochondrial mang（1518KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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