Effects of modified clay on the physiological and photosynthetic activities of Amphidinium carterae Hulburt

doi:10.1016/j.hal.2017.10.007

	Effects of modified clay on the physiological and photosynthetic activities of Amphidinium carterae Hulburt
	Liu, Shuya 1,2,3; Yu, Zhiming1,2,3 ; Song, Xiuxian1,2,3 ; Cao, Xihua1,2
	2017-12-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	70 页码:64-72
通讯作者	Yu, Zhiming([email protected])
摘要	Among the strategies for treating harmful algal blooms, flocculation using modified clay (MC) has been widely applied in the field. This paper studied the mitigation of MC on Amphidinium carterae Hulburt, finding that MC could not only effectively remove A. carterae, but also affect the physiological activities of the residual algae and inhibit their normal growth. The superoxide dismutase (SOD) activity, catalase (CAT) activity and malondialdehyde (MDA) content of the residual algae significantly increased compared with the control, indicating that MC stimulated the accumulation of reactive oxygen species (ROS) in algal cells. In addition, the cell density was significantly correlated with the SOD activity, CAT activity and MDA content in the experiment groups, suggesting that intracellular ROS might be the main internal factor inhibiting cell growth. To reveal the mechanism of ROSS generation, this paper further evaluated the effect of MC on photosynthesis in the residual microalgae, and found that compared with the control the absorption flux per photosystem II (PSII) reaction center (ABS/RC), the trapping flux per RC (TR0/RC) and the electron transport flux per RC (ET0/RC) increased, while the TR0/ABS and ET0/ABS decreased after adding 0.10 g/L and 0.25 g/L MC. These findings indicate that the MC led to an imbalance between photosynthetic light absorption and energy utilization and that the partial RCs became non primary quinone electron acceptor (Q(A))-RCs, further inducing the over-excitation of the active RCs. And MC caused the suppression of the electron transport chain (ETC): the ETC from the Q(A) to the secondary quinone electron acceptor (Q(B)) was blocked and the size of plastoquinone pool decreased, which could induce the over-reduction of PSII. The over-excitation of PSII and the damaged ETC likely induce the generation of ROS during photosynthesis. Thus, MC likely induced the accumulation of intracellular ROS due to photosynthesis inhibition, consequently hindering the growth of the residual algae. (C) 2017 Elsevier B.V. All rights reserved.
关键词	Modified clay Amphidinium carterae Hulburt Antioxidant enzyme Photosynthesis Chlorophyll a fluorescence
DOI	10.1016/j.hal.2017.10.007
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2017YFC1404306]###1272; AoShan Talents Cultivation Program[2017ASTCP-OS16]###1273; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404]###130; Chinese Academy of Sciences[XDA11020601]###1274; National Key Research and Development Program of China[2017YFC1404306]; AoShan Talents Cultivation Program[2017ASTCP-OS16]; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404]; Chinese Academy of Sciences[XDA11020601]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000418311500005
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157753
专题	海洋生态与环境科学重点实验室
通讯作者	Yu, Zhiming
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Liu, Shuya,Yu, Zhiming,Song, Xiuxian,et al. Effects of modified clay on the physiological and photosynthetic activities of Amphidinium carterae Hulburt[J]. HARMFUL ALGAE,2017,70:64-72.
APA	Liu, Shuya,Yu, Zhiming,Song, Xiuxian,&Cao, Xihua.(2017).Effects of modified clay on the physiological and photosynthetic activities of Amphidinium carterae Hulburt.HARMFUL ALGAE,70,64-72.
MLA	Liu, Shuya,et al."Effects of modified clay on the physiological and photosynthetic activities of Amphidinium carterae Hulburt".HARMFUL ALGAE 70(2017):64-72.