Comparison of pigment composition and melanin content among white, light-green, dark-green, and purple morphs of sea cucumber, Apostichopus japonicus

doi:10.1007/s13131-017-1056-5

	Comparison of pigment composition and melanin content among white, light-green, dark-green, and purple morphs of sea cucumber, Apostichopus japonicus
	Xing, Lili1,2 ; Sun, Lina 1; Liu, Shilin 1; Li, Xiaoni 1,2; Miao, Ting 3; Zhang, Libin 1; Yang, Hongsheng1
	2017-12-01
发表期刊	ACTA OCEANOLOGICA SINICA
ISSN	0253-505X
卷号	36 期号:12 页码:45-51
通讯作者	Yang, Hongsheng([email protected])
摘要	Sea cucumber, Apostichopus japonicus (Selenka), is a commercially important marine species in China. Among the differently colored varieties sold in China, white and purple sea cucumbers have the greatest appeal to consumers. Identification of the pigments that may contribute to the formation of different color morphs of sea cucumbers will provide a scientific basis for improving the cultivability of desirable color morphs. In this study, sea cucumbers were divided into four categories according to their body color: white, light green, dark green, and purple. The pigment composition and contents in the four groups were analyzed by high performance liquid chromatography (HPLC). The results show that the pigment contents differed significantly among the white, lightgreen, dark-green, and purple sea cucumbers, and there were fewer types of pigments in white sea cucumber than in the other color morphs. The only pigments detected in white sea cucumbers were guanine and pteroic acid. Guanine and pteroic acid are structural colors, and they were also detected in light-green, dark-green, and purple sea cucumbers. Every pigment detected, except for pteroic acid, was present at a higher concentration in purple morphs than in the other color morphs. The biological color pigments melanin, astaxanthin, beta-carotene, and lutein were detected in light-green, dark-green, and purple sea cucumbers. While progesterone and lycopene, which are also biological color pigments, were not detected in any of the color morphs. Melanin was the major pigment contributing to body color, and its concentration increased with deepening color of the sea cucumber body. Transmission electron microscopy analyses revealed that white sea cucumbers had the fewest epidermal melanocytes in the body wall, and their melanocytes contained fewer melanosomes as well as non-pigmented pre-melanosomes. Sea cucumbers with deeper body colors contained more melanin granules. In the body wall of dark-green and purple sea cucumbers, melanin granules were secreted out of the cell. The results of this study provide evidence for the main factors responsible for differences in coloration among white, light-green, darkgreen, and purple sea cucumbers, and also provide the foundation for further research on the formation of body color in sea cucumber, A. japonicus.
关键词	Apostichopus japonicas pigment composition color morphs melanin pigment content
DOI	10.1007/s13131-017-1056-5
收录类别	SCI
语种	英语
资助项目	Agricultural Seed Project of Shandong Province###83; Agricultural Seed Project of Shandong Province###83; Agricultural Seed Project of Shandong Province
WOS研究方向	Oceanography
WOS类目	Oceanography
WOS记录号	WOS:000417883300007
出版者	SPRINGER
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157380
专题	海洋生态与环境科学重点实验室
通讯作者	Yang, Hongsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Qingdao 266100, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Xing, Lili,Sun, Lina,Liu, Shilin,et al. Comparison of pigment composition and melanin content among white, light-green, dark-green, and purple morphs of sea cucumber, Apostichopus japonicus[J]. ACTA OCEANOLOGICA SINICA,2017,36(12):45-51.
APA	Xing, Lili.,Sun, Lina.,Liu, Shilin.,Li, Xiaoni.,Miao, Ting.,...&Yang, Hongsheng.(2017).Comparison of pigment composition and melanin content among white, light-green, dark-green, and purple morphs of sea cucumber, Apostichopus japonicus.ACTA OCEANOLOGICA SINICA,36(12),45-51.
MLA	Xing, Lili,et al."Comparison of pigment composition and melanin content among white, light-green, dark-green, and purple morphs of sea cucumber, Apostichopus japonicus".ACTA OCEANOLOGICA SINICA 36.12(2017):45-51.