ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection

doi:10.1016/j.fsi.2017.08.043

IOCAS-IR

	ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection
	Liu, Peng-fei1,2 ; Du, Yishuai 1; Meng, Lingjie1,2 ; Li, Xian 1; Liu, Ying1,3
	2017-11-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	70 页码:30-39
通讯作者	Liu, Ying([email protected])
摘要	Aldehyde dehydrogenases (ALDHs) belong to a super-family of detoxifying proteins and perform a significant role in developing epithelial homeostasis, protecting cells from toxic aldehydes and drug resistance. However, the activity and function of these detoxifying proteins remain unknown, especially in fish. In our research, we aimed to study functions of aldehyde dehydrogenase 7A1 (ALDH7A1) in Atlantic salmon infected by Aeromonas salmonicida. Recombinant ALDH7A1 (rALDH7A1) was verified by SDS-PAGE and western blot. The molecular mass of the deduced amino acid sequence of rALDH7A1 is 58.9 kDa with an estimated pI of 7.09. Only a low complexity region ((141)yvegvgevqeyvdv(153)) without a signal peptide existed in rALDH7A1. Results of ELISA indicated that rALDH7A1 exhibited apparent binding activities with A. salmonicida and its expression was highest in fish kidney. A Real Time PCR (qRT-PCR) assay in kidneys confirmed that fish in this experiment were authentically infected and bacterial loads in rALDH7A1-adminsitered fish were significantly reduced at an early stage of infection. Meanwhile, we found the mRNA expression of NF-k beta, P-38 MAPK, caspase-3 and TNF-alpha were mainly up regulated at 72 h in the kidneys and livers of highly infected fish injected with rALDH7A1, and the same variation trend existed in fish spleens at 12 h. Consistent with these observations, neutralization experiments in vivo indicated that rALDH7A1 could obviously reduce the death rate compared to the BSA and control group. Taken together, we concluded that rALDH7A1 could act in host immune defense against bacterial infection and decrease the mortality rate of Atlantic salmon at early stages of infection with A. salmonicida. (C) 2017 Elsevier Ltd. All rights reserved.
关键词	ALDH7A1 Aeromonas salmonicida Atlantic salmon Recombinant protein
DOI	10.1016/j.fsi.2017.08.043
收录类别	SCI
语种	英语
资助项目	National Natural Science Fund of China[41306152]###1224; National Natural Science Fund of China[31472312]###1225; National Natural Science Fund of China[31402283]###1226; key R&D Program of Jiangsu Province[BE2015325]###1227; earmarked fund for the Modern Agro-industry Technology Research System[CARS 48]###1228; National Natural Science Fund of China[41306152]###1224; National Natural Science Fund of China[31472312]###1225; National Natural Science Fund of China[31402283]###1226; key R&D Program of Jiangsu Province[BE2015325]###1227; earmarked fund for the Modern Agro-industry Technology Research System[CARS 48]###1228; National Natural Science Fund of China[41306152]; National Natural Science Fund of China[31472312]; National Natural Science Fund of China[31402283]; key R&D Program of Jiangsu Province[BE2015325]; earmarked fund for the Modern Agro-industry Technology Research System[CARS 48]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000416186800004
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157315
专题	中国科学院海洋研究所
通讯作者	Liu, Ying
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Dalian Ocean Univ, Dalian, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:30-39.
APA	Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,Li, Xian,&Liu, Ying.(2017).ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection.FISH & SHELLFISH IMMUNOLOGY,70,30-39.
MLA	Liu, Peng-fei,et al."ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection".FISH & SHELLFISH IMMUNOLOGY 70(2017):30-39.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S105046481730（4126KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览