Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos

doi:10.1186/1471-2121-11-70

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	Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos
	Bernick, Elena P.1,2; Zhang, Pei-Jun 2,3; Du, Shaojun 1,2
	2010-09-17
发表期刊	BMC CELL BIOLOGY
ISSN	1471-2121
卷号	11 页码:70
文章类型	Article
摘要	Background: Unc-45 is a myosin chaperone and a Hsp90 co-chaperone that plays a key role in muscle development. Genetic and biochemical studies in C. elegans have demonstrated that Unc-45 facilitates the process of myosin folding and assembly in body wall muscles. Loss or overexpression of Unc-45 in C. elegans results in defective myofibril organization. In the zebrafish Danio rerio, unc-45b, a homolog of C. elegans unc-45, is expressed in both skeletal and cardiac muscles. Earlier studies indicate that mutation or knockdown of unc-45b expression in zebrafish results in a phenotype characterized by a loss of both thick and thin filament organization in skeletal and cardiac muscle. The effects of unc-45b knockdown on other sarcomeric structures and the phenotype of Unc-45b overexpression, however, are poorly understood in vertebrates.; We thank John Stubblefield for editing, Junling Li for the assistance in the Western blot analysis. This research was supported by a training grant from National Institutes of Health (#T32 AR07592) and a research grant MB-8713-08 from United States - Israel Binational Agriculture Research and Development Fund.
DOI	10.1186/1471-2121-11-70
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000282734200001
引用统计	被引频次：57[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1571
专题	实验海洋生物学重点实验室
作者单位	1.Univ Maryland, Sch Med, Interdisciplinary Training Program Muscle Biol, Baltimore, MD 21201 USA 2.Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Bernick, Elena P.,Zhang, Pei-Jun,Du, Shaojun. Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos[J]. BMC CELL BIOLOGY,2010,11:70.
APA	Bernick, Elena P.,Zhang, Pei-Jun,&Du, Shaojun.(2010).Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos.BMC CELL BIOLOGY,11,70.
MLA	Bernick, Elena P.,et al."Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos".BMC CELL BIOLOGY 11(2010):70.

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