Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Quantitative Proteomics Reveals Common and Specific Responses o a Marine Diatom Thalassiosira pseudonana to Different Macronutrient Deficiencies | |
| Chen, Xiao-Huang1; Li, Yuan-Yuan1; Zhang, Hao1; Liu, Jiu-Ling1; Xie, Zhang-Xian1; Lin, Lin1; Wang, Da-Zhi1,2 | |
| 2018-11-14 | |
| 发表期刊 | FRONTIERS IN MICROBIOLOGY
 |
| ISSN | 1664-302X |
| 卷号 | 9页码:20 |
| 通讯作者 | Wang, Da-Zhi([email protected]) |
| 摘要 | Macronutrients such as nitrogen (N), phosphorus (P), and silicon (Si) are essential for the productivity and distribution of diatoms in the ocean. Responses of diatoms to a particular macronutrient deficiency have been investigated, however, we know little about their common or specific responses to different macronutrients. Here, we investigated the physiology and quantitative proteomics of a diatom Thalassiosira pseudonana grown in nutrient-replete, N-, P-, and Si-deficient conditions. Cell growth was ceased in all macronutrient deficient conditions while cell volume and cellular C content under P- and Si-deficiencies increased. Contents of chlorophyll a, protein and cellular N decreased in both N- and P-deficient cells but chlorophyll a and cellular N increased in the Si-deficient cells. Cellular P content increased under N-and Si-deficiencies. Proteins involved in carbon fixation and photorespiration were down-regulated under all macronutrient deficiencies while neutral lipid synthesis and carbohydrate accumulation were enhanced. Photosynthesis, chlorophyll biosynthesis, and protein biosynthesis were down-regulated in both N- and P-deficient cells, while Si transporters, light-harvesting complex proteins, chloroplastic ATP synthase, plastid transcription and protein synthesis were up-regulated in the Si-deficient cells. Our results provided insights into the common and specific responses of T. pseudonana to different macronutrient deficiencies and identified specific proteins potentially indicating a particular macronutrient deficiency. |
| 关键词 | marine diatom Thalassiosira pseudonana macronutrient nitrogen phosphorus silicon quantitative proteomics |
| DOI | 10.3389/fmicb.2018.02761 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[41230961]###295; National Natural Science Foundation of China[41425021]###297; National Key R&D Program of China[2017YFC1404300]###294; Ten Thousand Talents Program for leading talents in science and technological innovation###841; National Natural Science Foundation of China[41230961]; National Natural Science Foundation of China[41425021]; National Key R&D Program of China[2017YFC1404300]; Ten Thousand Talents Program for leading talents in science and technological innovation |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| WOS记录号 | WOS:000450087500001 |
| 出版者 | FRONTIERS MEDIA SA |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/156373 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Wang, Da-Zhi |
| 作者单位 | 1.Xiamen Univ, State Key Lab Marine Environm Sci, Coll Environm & Ecol, Xiamen, Peoples R China 2.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao, Peoples R China |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Xiao-Huang,Li, Yuan-Yuan,Zhang, Hao,et al. Quantitative Proteomics Reveals Common and Specific Responses o a Marine Diatom Thalassiosira pseudonana to Different Macronutrient Deficiencies[J]. FRONTIERS IN MICROBIOLOGY,2018,9:20. |
| APA | Chen, Xiao-Huang.,Li, Yuan-Yuan.,Zhang, Hao.,Liu, Jiu-Ling.,Xie, Zhang-Xian.,...&Wang, Da-Zhi.(2018).Quantitative Proteomics Reveals Common and Specific Responses o a Marine Diatom Thalassiosira pseudonana to Different Macronutrient Deficiencies.FRONTIERS IN MICROBIOLOGY,9,20. |
| MLA | Chen, Xiao-Huang,et al."Quantitative Proteomics Reveals Common and Specific Responses o a Marine Diatom Thalassiosira pseudonana to Different Macronutrient Deficiencies".FRONTIERS IN MICROBIOLOGY 9(2018):20. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Quantitative Proteom（4835KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论