Fucoxanthin Exerts Cytoprotective Effects against Hydrogen Peroxide-induced Oxidative Damage in L02 Cells

doi:10.1155/2018/1085073

IOCAS-IR > 实验海洋生物学重点实验室

	Fucoxanthin Exerts Cytoprotective Effects against Hydrogen Peroxide-induced Oxidative Damage in L02 Cells
	Wang, Xia1,2 ; Cui, Yan-jun 3; Qi, Jia 3; Zhu, Min-min 3; Zhang, Tian-liang 4; Cheng, Min 5; Liu, Shun-mei 3,4; Wang, Guang-ce 6
	2018
发表期刊	BIOMED RESEARCH INTERNATIONAL
ISSN	2314-6133
页码	11
通讯作者	Liu, Shun-mei([email protected]) ; Wang, Guang-ce([email protected])
摘要	Several previous studies have demonstrated the excellent antioxidant activity of fucoxanthin against oxidative stress which is closely related to the pathogenesis of liver diseases. The present work was to investigate whether fucoxanthin could protect human hepatic L02 cells against hydrogen peroxide-(H2O2-) induced oxidative damage. Its effects on H2O2- induced cell viability, lactate dehydrogenase (LDH) leakage, intracellular reduced glutathione, and reactive oxygen species (ROS) contents, along with mRNA and protein relative levels of the cytoprotective genes including Nrf2, HO-1, and NQO1, were investigated. The results showed that fucoxanthin could upregulate the mRNA and protein levels of the cytoprotective genes and promote the nuclear translocation of Nrf2, which could be inhibited by the PI3K inhibitor of LY294002. Pretreatment of fucoxanthin resulted in decreased LDH leakage and intracellular ROS content but enhanced intracellular reduced glutathione. Interestingly, pretreatment using fucoxanthin protected against the oxidative damage in a nonconcentration-dependent manner, with fucoxanthin of 5 mu M demonstrating the optimal effects. The results suggest that fucoxanthin exerts cytoprotective effects against H2O2-induced oxidative damage in L02 cells, which may be through the PI3K-dependent activation of Nrf2 signaling.
DOI	10.1155/2018/1085073
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81870237]###770; Shandong Provincial Natural Science Foundation, China[ZR2010DM010]###771; Shandong Provincial Natural Science Foundation, China[ZR2017LC021]###772; Higher Educational Science and Technology Program of Shandong Province[J10LC20]###773; National Natural Science Foundation of China[81870237]; Shandong Provincial Natural Science Foundation, China[ZR2010DM010]; Shandong Provincial Natural Science Foundation, China[ZR2017LC021]; Higher Educational Science and Technology Program of Shandong Province[J10LC20]
WOS研究方向	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS类目	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS记录号	WOS:000451147600001
出版者	HINDAWI LTD
引用统计	被引频次：41[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/156169
专题	实验海洋生物学重点实验室
通讯作者	Liu, Shun-mei; Wang, Guang-ce
作者单位	1.Weifang Med Univ, Sch Publ Hlth & Management, Weifang 261053, Peoples R China 2.Weifang Med Univ, Collaborat Innovat Ctr Predict & Governance Major, Weifang 261053, Peoples R China 3.Weifang Med Univ, Sch Biosci & Technol, Weifang 261053, Peoples R China 4.Weifang Med Univ, Expt Ctr Med Res, Weifang 261053, Peoples R China 5.Weifang Med Univ, Sch Clin Med, Weifang 261053, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xia,Cui, Yan-jun,Qi, Jia,et al. Fucoxanthin Exerts Cytoprotective Effects against Hydrogen Peroxide-induced Oxidative Damage in L02 Cells[J]. BIOMED RESEARCH INTERNATIONAL,2018:11.
APA	Wang, Xia.,Cui, Yan-jun.,Qi, Jia.,Zhu, Min-min.,Zhang, Tian-liang.,...&Wang, Guang-ce.(2018).Fucoxanthin Exerts Cytoprotective Effects against Hydrogen Peroxide-induced Oxidative Damage in L02 Cells.BIOMED RESEARCH INTERNATIONAL,11.
MLA	Wang, Xia,et al."Fucoxanthin Exerts Cytoprotective Effects against Hydrogen Peroxide-induced Oxidative Damage in L02 Cells".BIOMED RESEARCH INTERNATIONAL (2018):11.

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