A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas

doi:10.1016/j.dci.2018.10.014

IOCAS-IR > 实验海洋生物学重点实验室

	A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas
	Liu, Rui 1; Cheng, Qi 1,4; Song, Xiaorui2,3,5 ; Wang, Hao 1; Wang, Xiudan1 ; Wang, Lingling 3,5; Zhu, Beiwei 4; Song, Linsheng 2,3,5
	2019-02-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	91 页码:132-142
通讯作者	Wang, Hao() ; Song, Linsheng([email protected])
摘要	As an important post-translational protein modification, ubiquitination has been demonstrated to play vitalrole in immune response of vertebrates. Ubiquitin (Ub)-conjugating enzyme E2 is the "heart" of ubiquitination, which is responsible for Ub cellular signaling and substrate modification. In the present study, an Ub-conjugating enzyme E2 (designed as CgUbe2g1) was identified from oyster Crassostrea gigas, and its regulation in the immune response against lipopolysaccharide (LPS) stimulation was investigated. CgUbe2g1 encoded a polypeptide of 168 amino acids with the predicted molecular mass of 19.20 kDa and contained conserved catalytic 'Ube domains. It shared a higher similarity with the known UBC2G1 type E2s and was closely clustered with the type E2s identified from invertebrates in the phylogenetic assay. The mRNA transcripts of CgUbe2g1 were mainly distributed in hemocyte, mantle, hepatopancreas and male gonad of C. gigas. CgUbe2g1 protein was found to be colocalized with Ub around the nucleus of oyster hemocyte. The recombinant CgUbe2g1 protein (rCgUbe2g1) could activate the ubiquitination in vitro by binding both activated and un-activated Ub. The expressions of inflammation-related factors TNF-alpha and NF-kappa B in CgUbe2g1 transfected cells were both significantly up-regulated after LPS stimulation, which were 12.9-fold at 3h (p < 0.01) and 2.3-fold at 6h (p < 0.01) of that in negative control group, respectively. The phagocytic rate of hemocyte and the ROS level in hemocyte were both significantly decreased (p < 0.01), while the apoptosis rate was significantly increased (p < 0.01) after CgUbe2g1 mRNA was interfered. These results demonstrated that Ub-conjugating enzyme CgUbe2g1 was involved in the innate immune response of oyster against invading pathogen, which might play important roles in the activation of inflammatory response and regulation of cellular immune response.
关键词	Ub-conjugating enzyme E2 Inflammatory response Cellular immunity Crassostrea gigas NF-kappa B pathway Ubiquitination
DOI	10.1016/j.dci.2018.10.014
收录类别	SCI
语种	英语
资助项目	National Science Foundation of China[U1706204]###733; Aoshan Talent Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13]###739; Modern Agro-industry Technology Research System, Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture[CARS-49]###740; Research Foundation for Distinguished Professor in Liaoning###737; Talented Scholars in Dalian Ocean University###741; Dalian High Level Talent Innovation Support Program[2015R020]###742; National Science Foundation of China[U1706204]; Aoshan Talent Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13]; Modern Agro-industry Technology Research System, Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture[CARS-49]; Research Foundation for Distinguished Professor in Liaoning; Talented Scholars in Dalian Ocean University; Dalian High Level Talent Innovation Support Program[2015R020]
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000451492900015
出版者	ELSEVIER SCI LTD
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/156148
专题	实验海洋生物学重点实验室
通讯作者	Wang, Hao; Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Dalian Univ Technol, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 4.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China 5.Dalian Univ Technol, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Liu, Rui,Cheng, Qi,Song, Xiaorui,et al. A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,91:132-142.
APA	Liu, Rui.,Cheng, Qi.,Song, Xiaorui.,Wang, Hao.,Wang, Xiudan.,...&Song, Linsheng.(2019).A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,91,132-142.
MLA	Liu, Rui,et al."A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 91(2019):132-142.