Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection

doi:10.1016/j.dci.2018.11.006

IOCAS-IR > 实验海洋生物学重点实验室

	Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection
	Zhang, Jian 1,2,3; Sun, Li 1,2
	2019-03-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	92 页码:150-159
通讯作者	Sun, Li([email protected])
摘要	Megalocytivirus is a DNA virus with a broad host range among farmed fish including tongue sole (Cynoglossus semilaevis). In this study, label-free proteomics was performed to examine protein expression in tongue sole spleen induced by megalocytivirus at 8 and 12 days post infection (dpi). Compared to uninfected control fish, virus-infected fish displayed 315 up-regulated proteins and 111 down-regulated proteins at 8 dpi, and 48 up regulated proteins and 43 down-regulated proteins at 12 dpi. The expressions of five differentially expressed proteins were confirmed by Western blot. The differentially expressed proteins were enriched in the pathways and processes associated with innate immune response and viral infection. Interference with the expression of two up-regulated proteins of the ubiquitin proteasome system (UPS), i.e. proteasome assembly chaperone 2 and proteasome maturation protein, significantly reduced viral propagation in fish, whereas overexpression of these two proteins significantly enhanced viral propagation. Consistently, inhibition of the functioning of proteasome significantly impaired viral replication in vivo. This study provided the first global protein profile responsive to megalocytivirus in tongue sole, and revealed an essential role of UPS in viral infection.
关键词	Cynoglossus semilaevis Megalocytivirus Viral infection Immune defense Ubiquitin proteasome system
DOI	10.1016/j.dci.2018.11.006
收录类别	SCI
语种	英语
资助项目	Taishan Scholar Program of Shandong Province###423; Qingdao National Laboratory for Marine Science and Technology[2015ASTP]###289; Aoshan Talents Program###288; National Natural Science Foundation of China[31730100]###519; National Natural Science Foundation of China[31502197]###518; National Natural Science Foundation of China[31502197]; National Natural Science Foundation of China[31730100]; AoShan Talents Program; Qingdao National Laboratory for Marine Science and Technology[2015ASTP]; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000458596200016
出版者	ELSEVIER SCI LTD
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/155595
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Deep Sea Res Ctr, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室; 中国科学院海洋研究所
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Jian,Sun, Li. Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,92:150-159.
APA	Zhang, Jian,&Sun, Li.(2019).Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,92,150-159.
MLA	Zhang, Jian,et al."Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 92(2019):150-159.

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