Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei | |
Du, Yang1,2; Zhou, Shuhong2,3; Liu, Mei1,2; Wang, Baojie1,2; Jiang, Keyong1,2; Fang, Han2,3 ; Wang, Lei1,2,4
| |
| 2019-03-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| 卷号 | 86页码:1194-1206 |
| 通讯作者 | Wang, Lei([email protected]) |
| 摘要 | The interactions of microbiota in the intestines play an important role in promoting or maintaining the health of hosts. The present study aim to investigate the effects of the surface proteins of Lactobacillus pentosus HC-2 on the immune response and the bacterial composition of Litopenaeus vannamei, thus, the immune -related genes, surface condition, HC-2 numbers and the bacteria diversity in midgut were explored after shrimp feeding the normal HC-2 and 5 M - lithium chloride (LiCI) treated HC-2 for four weeks. Obvious improvements in the intestinal surface were observed in R group than the control group and L group. qPCR analysis demonstrated that the selected immune -related genes of lysozyme, proPO, LGBP, PEN-3 alpha, crustin, and lvLec were significantly up-regulated in group R than in group L Meanwhile, in the challenge test, shrimp in R group received 72% relative percent survival, which was significantly higher than the L group (RPS = 9%). The bacteria composition analysis showed that the abundance of Proteobacteria were significantly higher in group R and L than in group C, and the Bacteroidetes were significantly higher in group C than in group R and L, whereas the numbers of Chloroflexi were significantly higher in group R than in group C and L. The bacterial community difference analysis revealed that the harmful bacteria such as genus of Vibrio, Tenacibaculu and Thalassobius were decreased and the beneficial bacterium as Ruegeria and Lactobacillus were increased in R group, whereas this phenomenon were not found in L group. Taken together, above results indicating that the surface proteins were indispensable for L. pentosus HC-2 adhesion and colonization in shrimp intestines to improve intestine condition, enhance immune response, competitively exclude the pathogens, and promote the beneficial bacteria growth to protect the shrimp from pathogens infection. The findings in this work will help to promote the understanding of the roles of probiotics in shrimp intestines displaying probiotic-function by regulating the intestinal bacteria. |
| 关键词 | Litopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins Immune response Gut bacteria diversity |
| DOI | 10.1016/j.fsi.2018.12.073 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | China Postdoctoral Science Foundation[2018M632736]###395; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH]###394; National Natural Science Foundation of China[31802309]###393; National Natural Science Foundation of China[31802309]; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH]; China Postdoctoral Science Foundation[2018M632736] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000459528400132 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/155394 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Du, Yang,Zhou, Shuhong,Liu, Mei,et al. Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:1194-1206. |
| APA | Du, Yang.,Zhou, Shuhong.,Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,...&Wang, Lei.(2019).Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,86,1194-1206. |
| MLA | Du, Yang,et al."Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 86(2019):1194-1206. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Understanding the ro（3289KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论