The Effect of Melatonin on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus

doi:10.3389/fphys.2019.00221

	The Effect of Melatonin on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus
	Ding, Kui1,2,3,4 ; Zhang, Libin 1,2,3,4; Zhang, Tao1,2,3,4 ; Yang, Hongsheng1,2,3,4 ; Brinkman, Richard 5
	2019-03-19
发表期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
卷号	10 页码:13
摘要	Melatonin is a highly conserved hormone in evolutionary history. It occurs in numerous organisms and plays a role in the endocrine and immune systems. Locomotor behavior is a basic behavior in animals and is an important indicator of circadian rhythms, which are coordinated by the nervous and endocrine systems. To date, the effect of melatonin on locomotor behavior has been studied in vertebrates, including syrian hamsters, sparrows, rats, zebrafish, goldfish, and flatworms. However, there have been few studies of the effects of melatonin on locomotor behavior in marine invertebrates. The goals of present study were to show the existence of melatonin in the sea cucumber Apostichopus japonicus and to evaluate its effect on locomotor activity. In addition, muscle tissues from control and melatonin-treated sea cucumbers were tested using ultra performance liquid chromatography and quadrupole time-offlight mass spectrometry (UPLC-Q-TOF-MS) to determine the changes of metabolic activity in muscle. Melatonin was present in the coelomic fluid of A. japonicus at a concentration of similar to 135.0 ng/L. The total distance traveled and number steps taken over 9 h after melatonin administration decreased with increasing concentration of the melatonin dose. Mean and maximum velocity of movement and stride length and stride frequency also decreased, but their differences were not statistically significant. Overall, these results suggest that melatonin administration had a sedative effect on A. japonicus. The levels of 22 different metabolites were altered in the muscle tissues of melatonin-treated sea cucumbers. Serotonin, 9-cis retinoic acid, all-trans retinoic acid, flavin mononucleotide in muscles were downregulated after melatonin administration. Moreover, a high free fatty acid (FFA) concentration and a decrease in the adenosine 5'-triphosphate (ATP) concentration in the muscle tissues of the melatonin-treated group were detected as well. These results suggest that the sedative effect of melatonin involves some other metabolic pathways, and the reduced locomotor modulator- serotonin, inhibited fatty acid oxidation and disturbed oxidative phosphorylation are potential physiological mechanisms that result in the inhibitory effect of melatonin on locomotion in sea cucumbers.
关键词	melatonin sea cucumber locomotor behavior physiological mechanism fatty acid oxidation oxidative phosphorylation
DOI	10.3389/fphys.2019.00221
收录类别	SCI
语种	英语
资助项目	Taishan Scholars Program###321; Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]###320; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science###319; Funding of Youth Innovation Promotion Association, CAS[2015163]###318; National Key Research and Development Plan[2016YFE0101500]###317; National Natural Science Foundation of China[41676136]###81; National Natural Science Foundation of China[41676136]; National Key Research and Development Plan[2016YFE0101500]; Funding of Youth Innovation Promotion Association, CAS[2015163]; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science; Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]; Taishan Scholars Program
WOS研究方向	Physiology
WOS类目	Physiology
WOS记录号	WOS:000461732100001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/155286
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Libin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Australian Inst Marine Sci, Townsville, Qld, Australia
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ding, Kui,Zhang, Libin,Zhang, Tao,et al. The Effect of Melatonin on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus[J]. FRONTIERS IN PHYSIOLOGY,2019,10:13.
APA	Ding, Kui,Zhang, Libin,Zhang, Tao,Yang, Hongsheng,&Brinkman, Richard.(2019).The Effect of Melatonin on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus.FRONTIERS IN PHYSIOLOGY,10,13.
MLA	Ding, Kui,et al."The Effect of Melatonin on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus".FRONTIERS IN PHYSIOLOGY 10(2019):13.

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