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Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics | |
Zhang, Shu-Feng1; Chen, Ying1; Xie, Zhang-Xian1; Zhang, Hao1; Lin, Lin1; Wang, Da-Zhi1,2 | |
2019-03-30 | |
发表期刊 | JOURNAL OF PROTEOMICS
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ISSN | 1874-3919 |
卷号 | 196页码:141-149 |
通讯作者 | Wang, Da-Zhi([email protected]) |
摘要 | Phosphorus (P) is a key macronutrient limiting cell growth and bloom formation of marine dinoflagellates. Physiological responses to changing ambient P have been investigated in dinoflagellates; however, the molecular mechanisms behind these responses remain limited. Here, we compared the protein expression profiles of a marine dinoflagellate Alexandrium catenella grown in inorganic P-replete, P-deficient, and inorganic- and organic-P resupplied conditions using an iTRAQ-based quantitative proteomic approach. P deficiency inhibited cell growth and enhanced alkaline phosphatase activity (APA) but had no effect on photosynthetic efficiency. After P resupply, the P-deficient cells recovered growth rapidly and APA decreased. Proteins involved in sphingolipid metabolism, organic P utilization, starch and sucrose metabolism, and photosynthesis were up-regulated in the P-deficient cells, while proteins associated with protein synthesis, nutrient assimilation and energy metabolism were down-regulated. The responses of the P-deficient A. catenella to the resupply of organic and inorganic P presented significant differences: more biological processes were enhanced in the organic P-resupplied cells than those in the inorganic P-resupplied cells; A. catenella might directly utilize G-6-P for nucleic acid synthesis through the pentose phosphate pathway. Our results indicate that A. catenella has evolved diverse adaptive strategies to ambient P deficiency and specific mechanisms to utilize dissolved organic P, which might be an important reason resulting in A. catenella bloom in the low inorganic P environment. Biological significance: The ability of marine dinoflagellates to utilize different phosphorus (P) species and adapt to ambient P deficiency determines their success in the ocean. In this study, we investigated the response mechanisms of a dinoflagellate Alexandrium catenella to ambient P deficiency, and resupply of inorganic- and organic-P at the proteome level. Our results indicated that A. catenella initiated multiple adaptive strategies to ambient P deficiency, e.g. utilizing nonphospholipids and glycosphingolipids instead of phospholipids, enhancing expression of acid phosphatase to utilize organic P, and reallocating intracellular energy. Proteome responses of the P-deficient A. catenella to resupply of inorganic- and organic-P differed significantly, indicating different utilization pathways of inorganic and organic P, A. catenella might directly utilize low molecular weight organic P, such as G-6-P as both P and carbon sources. |
关键词 | Marine dinoflagellates Harmful algal blooms Alexandrium catenella Phosphorus Quantitative proteomics |
DOI | 10.1016/j.jprot.2018.11.004 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | 'Ten Thousand Talents Program' for leading talents in science and technological innovation###298; National Natural Science Foundation of China[41425021]###297; National Natural Science Foundation of China[41706131]###296; National Natural Science Foundation of China[41230961]###295; National Key R&D Program of China[2017YFC1404300]###294; National Key R&D Program of China[2017YFC1404300]; National Natural Science Foundation of China[41230961]; National Natural Science Foundation of China[41706131]; National Natural Science Foundation of China[41425021]; 'Ten Thousand Talents Program' for leading talents in science and technological innovation |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemical Research Methods |
WOS记录号 | WOS:000460716800013 |
出版者 | ELSEVIER SCIENCE BV |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/155271 |
专题 | 海洋生态与环境科学重点实验室 |
通讯作者 | Wang, Da-Zhi |
作者单位 | 1.Xiamen Univ, Coll Environm & Ecol, State Key Lab Marine Environm Sci, Xiamen 361102, Fujian, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Shu-Feng,Chen, Ying,Xie, Zhang-Xian,et al. Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics[J]. JOURNAL OF PROTEOMICS,2019,196:141-149. |
APA | Zhang, Shu-Feng,Chen, Ying,Xie, Zhang-Xian,Zhang, Hao,Lin, Lin,&Wang, Da-Zhi.(2019).Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics.JOURNAL OF PROTEOMICS,196,141-149. |
MLA | Zhang, Shu-Feng,et al."Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics".JOURNAL OF PROTEOMICS 196(2019):141-149. |
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